A total of 98 blood samples were genotyped by sequencing of the FUT2 gene.
A FUT2 gene common polymorphism determines resistance to rotavirus A of the P genotype.
The para-Bombay blood group is caused by a deficient FUT1 (191), whereas the non-secretor blood group is caused by a deficient FUT2
A region of the FUT2
gene was amplified, by using the following primers: forward 5'-CCCATCTTCAGAATCACCCTGCCGGTGCTG-3' and reverse 5'-TCGGCCGGCCCGTGGAAACATCCCC AGGTA-3', which anneal at positions 280-309 and 535-564, respectively, as described (18).
a] structure is complex and involves at least three genes, FUT3, FUT2
, and a sialyltransferase gene, that interact and compete with each other to produce the final products (Fig.
A single nucleotide polymorphism at position 428 in the FUT2
gene was investigated by pyrosequencing (3,11).
The recent cloning of the secretor gene (FUT2
) and the identification of enzyme-inactivating mutations and gene fusion [25-30] may even make it possible to classify the secretors as homozygous wild-type and heterozygous at the FUT2
locus, once the geographical distribution of inactivating mutations has been documented.
We obtained 38 saliva samples from healthy persons with ABO, Secretor, and Lewis phenotypes, and we selected confirmed FUT2
(secretor) and FUT3 (Lewis) genotypes (4) to include ABO, secretor, and Lewis phenotypic diversity.
Several studies (6-11) have associated norovirus susceptibility with the presence of an [alpha]1,2-linked fucose on histo-blood group antigens (HBGAs), which is determined by the FUT2