In this study, we prepared differentiating cells derived from hES cell (d-hES) for use as a feeder cells
of embryo co-culture.
The company adds a defined PICM-19H culture system (one free of feeder cells
and undefined cell culture medium constituents) may also be important for potential US Food and Drug Administration (FDA) approval of HepaLife's artificial liver device currently under development.
hiPSCs are usually maintained on inactivated Mouse Embryonic Fibroblasts (MEFs) as a feeder, but applying non-human feeder cells
can produce biological contaminants such as exogenous antigens, viruses and pathogens.
We do not know if feeder cells
were used, and whether they are of human or animal origin.
In addition, to provide physical support for stem cell attachment, feeder cells
also affect stem cells by producing various undefined factors through their interactions with stem cells (8).
By studying the differences in genes expressed in cultured mouse iPS cells between cases in which feeder cells
are used and those with large amounts of the LIF protein given without feeder cells
, they found the CCL2 is also associated with maintaining the pluripotency of iPS cells.
The use of feeder cells
in cell culture was first reported by Puck et al.
These feeder cells
offer embryonic stem cells a suitable attachment surface and also release largely uncharacterized nutrients into the culture medium that support stem cell growth in the undifferentiated state.
Stem Cells published Geron studies which demonstrated that human embryonic stem cells (hESCs) can be propagated in culture using defined growth factors without the need for feeder cells
or media conditioned by feeder cells
Most of the time--with only a few exceptions--hESCs are cultured in the presence of mouse feeder cells
Critics say the lines are aging and may be contaminated with mouse feeder cells