Fehling's solution(redirected from Fehling reagent)
Also found in: Dictionary, Thesaurus, Medical.
Fehling's solution(fā`lĭngz), deep-blue, alkaline solution used to test for the presence of aldehydesaldehyde
[alcohol + New Lat. dehydrogenatus=dehydrogenated], any of a class of organic compounds that contain the carbonyl group, , and in which the carbonyl group is bonded to at least one hydrogen; the general formula for an aldehyde is RCHO, where R is hydrogen
..... Click the link for more information. (e.g., formaldehyde, HCHO) or other compounds that contain the aldehyde functional group, -CHO. The substance to be tested is heated with Fehling's solution; formation of a brick-red precipitate indicates the presence of the aldehyde group. Simple sugars (e.g., glucose) give a positive test, so the solution has been used to test for the presence of glucose in urine, a symptom of diabetes; Benedict's solutionBenedict's solution,
deep-blue alkaline solution used to test for the presence of the aldehyde functional group, −CHO. The substance to be tested is heated with Benedict's solution; formation of a brick-red precipitate indicates presence of the aldehyde group.
..... Click the link for more information. , which gives the same test, is now more widely used. Fehling's solution is prepared just before use by mixing equal volumes of two previously prepared solutions, one containing about 70 grams cupric sulfate pentahydrate per liter of solution and the other containing about 350 grams Rochelle salt (potassium sodium tartrate tetrahydrate) and 100 grams sodium hydroxide per liter of solution. The cupric ion (complexed with tartrate ion) is reduced to cuprous ion by the aldehyde (which is oxidized) and precipitates as cuprous oxide (Cu2O); for this reason, sugars that react with Fehling's solution are called reducing sugars.
The Columbia Electronic Encyclopedia™ Copyright © 2013, Columbia University Press. Licensed from Columbia University Press. All rights reserved. www.cc.columbia.edu/cu/cup/