fermenter

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fermenter

[fər′ment·ər]
(food engineering)
A vessel used for fermenting, such as a vat for fermenting mash in brewing.
References in periodicals archive ?
The probes (Table 1), the biotinylated universal primers (Conjugate control) and the PCR products from non-biotinylated universal primers (Amplification control), were prepared with concentrations of 2.5 pmol/[micro]d; they were then spotted onto SensiBlot[TM] Plus Nylon membranes (Fermentas GmbFI, Germany) employing a 96-well Dot-Slot Blotter (Cleaver, UK) according to the manufacturer protocol.
Hundred uL of reaction mixture was made up of 7 uL dNTPs, 5 uL of 10x PCR buffer, 3 uL MgCl2, 2 uL of each primer, 2.0 uLTaq polymerase (Fermentas, USA), 10 uL template DNA and 10 mMTris buffer (pH 8.3) to make the volume.
Restriction digestion was done at 37[degrees]C for 5 minutes using 5 units of Fok-I fast digest enzyme (Fermentas, USA).
Next, 100 [micro]L of RCA mix [0.5 U/[micro]L Phi29 polymerase (Fermentas) in 1X Phi29 polymerase buffer (Fermentas) with 0.25 g/L BSA, 0.4 mmol/L dNTPs (Fermentas), and ddH2O] was added, followed by incubation for 1-1.5 h at 37[degrees]C and then by washes.
Further RNA contamination was removed by RNase treatment (Fermentas U.K.) for one hour at 37[degrees]C.
At specified time-points, MTB infected RAW264.7 cells were harvested and total RNA isolation was carried out using GeneJET[TM] RNA purification kit (Fermentas).
Genomic DNA samples (250 ng) were digested with FastDigest EcoRI and subsequently with FastDigest Taql (Fermentas, Hanover, MD, USA) based on the manufacturer's instructions.
The PCR products were analyzed on 1.5% agarose gel and purified by GeneJET[TM] Gel Extraction kit K0691 (Fermentas).
Lane M: 100 bp DNA ladder (Fermentas, USA); Lane 1: negative control (IC); Lane 2: PCR amplicon of K.