Xu J: Preliminary survival studies on autologous cultured skin fibroblasts
transplantation by injection.
gingivalis LPS at a concentration of 10 [micro]g/mL, gingival fibroblasts
produced a significantly increased level of TGF-[beta] when compared to control (Figure 1).
The previous study ( in vivo study) conducted by our group demonstrated the isolation of fibroblasts
from the uterosacral ligament tissues of POP and non-POP (NPOP) patients by laser capture microdissection.
Passage 3 fibroblasts
began to adhere after seeding for 2 h.
were cultured to 80% to 90% confluence on 150-mm plates.
However, unexpectedly, it has been recently observed that MUC1 mucin was expressed by normal human skin fibroblasts
, which were assumed as negative control during authors' experiments .
The researchers transplanted the cardiac fibroblasts
isolated from the region of calcification under the skin of healthy mice and observed soft-tissue calcification similar to that seen in the donor mice.
cells, isolated from healthy hair follicles, are a rich source of fibroblasts
unique in their high-level expression of the necessary proteins, such as Type I collagen, required to jump-start the stalled healing cycle.
Objective: To determine the effect of fibroblast
growth factor 2 on cognitive function in neonatal rats with hypoxic- ischaemic brain injury.
form a meshwork of fibronectin that helps support cells.
Primary lung fibroblasts
were harvested from these animals and cultured in DMEM supplemented with 10% FBS and 1% antibiotic-antimycotic solution (Cellgro, Manassas, VA) as previously described [31, 32].
Caption: Figure 1: Isolation and identification of human fibroblasts
and ASCs in vitro.