luciferase

(redirected from Firefly luciferase)
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luciferase

[lü′sif·ə‚rās]
(biochemistry)
An enzyme that catalyzes the oxidation of luciferin.
References in periodicals archive ?
HUVEC plated in 24-well plate were cotransfected with NC or miR-106b-5p mimic (final concentration: 60 pmol/ml, Life Technology) and firefly luciferase reporter plasmid (final concentration: 300 ng/ml) along with renilla luciferase control plasmid (final concentration: 10 ng/ml; Promega) using lipofectamine 2000 (final concentration: 4 mg/ml, Promega).
Borghei and Hall (2014) used firefly luciferase to develop a red-shifted ATP sensor.
MAbs recognizing FFL(Firefly luciferase) specificly were developed by obtaining hybridoma cell lines from the immunized animals injected with the firefly luciferase protein .
The luciferase activity resulting from the various ovine IFNT-reporter constructs was normalized by dividing the luminescence of firefly luciferase by that of Renilla luciferase (Imakawa et al.
Several reporter genes have been effectively used in biological screens including green fluorescent protein (GFP), chloramphenicol acetyl transferase (CAT), [beta]-galactosidase, firefly luciferase, and alkaline phosphatase (30).
Microdetermination of adenosine diphosphate and adenosine triphosphate in plasma with firefly luciferase system.
With P450-Glo, light is generated de novo by firefly luciferase, obviating the problem of high background signals related to the excitation light used in fluorescent assays.
The resulting transfectants expressed generally increased, but variable levels of firefly luciferase from the construct containing the GA sequence, at a pH of 6.
Frustrated by these difficulties, Hall asked Kay whether they could join the firefly luciferase gene to per and simply watch the clock gene in action.
Values for extract-treated cells were considered as hits if firefly luciferase activity was >125% and Renilla luciferase activity <125% of solvent-treated cells and if at least for four out of five experiments effect sizes were similar.