luciferase

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luciferase

[lü′sif·ə‚rās]
(biochemistry)
An enzyme that catalyzes the oxidation of luciferin.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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Although the performance of the latter for cell tracking has been evaluated in vivo [14], no attempts have yet been made to combine either reporter with firefly luciferase in order to establish protocols for the coimaging of multiple cell populations.
Bcl-2-expressing CD-NSCs survived longer than native CD-NSCs when transplanted into the brains of immunodeficient mice, as evidenced by prolonged firefly luciferase signal and significantly reduced active caspase-3 staining.
Our results showed that most of the extracts/fractions and negative controls presented low firefly luciferase expression and, therefore, a low firefly/Renilla ratio (Figure 4(a)).
To establish a reporter gene construct that responds optimally to ligation of the Fc[gamma]RIIIA receptor (CD16a), a series of functional reporter gene constructs consisting of the recognition sequences for individual transcription factors implicated in CD16a signal transduction regulating expression of the firefly luciferase (FL) reporter gene was used to determine the response of a given transcription factor during an ADCC reaction.
Firefly luciferase and its exogenously supplied substrate, luciferin, can be used to measure ATP because the chemiluminescent reaction is ATP-dependent.
These amplicons were sequenced and cloned into a firefly luciferase reporter vector [pGL4.23 (luc2/minP), Promega Corp.] that is digested with Kpnl and Hindlll using an In-Fusion HD Cloning Kit (Clontech Laboratories, Inc., CA, USA).
HEK293T/17 cells were transfected with plasmids encoding a yeast GAL4 DNA-binding domain-mouse PPAR[gamma] ligand-binding domain fusion protein, a yeast UAS (GAL4 upstream activator sequence) driving firefly luciferase, and a constitutively active Renilla luciferase transfection efficiency control.
Cells were plated on 96 well plates prior to performing transient transfection.24 h after plating, HEK293T cells were cotransfected with 100 ng ofNgn3 expression vector and pGL3-basic vector containing a 2399-bp portion of the Sohlh1promoter region upstream of the firefly luciferase gene (pGL3-sohlh1A Luc) plasmid, using Lipofectamine-2000 reagent (Invitrogen) in Opti- MEM(Minimum Essential Media) (Invitrogen).
The activities of firefly luciferase and Renilla luciferase were sequentially determined using a luminescence microplate reader (Infinite(R)200; Tecan Japan Co.
By cloning the 3'UTR of TET2 containing these sites downstream of the firefly luciferase gene in the pMIR-REPORT reporter vector, the resultant plasmid was able to be co-transfected with a mimic of miR-29 or a scrambled miRNA control.
FFL(Firefly luciferase) catalyzes the oxidation of luciferin in the presence of ATP, Mg2+ and molecular oxygen to produce light, oxyluciferin, CO2 and AMP [1].