fluorescent in situ hybridization

(redirected from Fluorescence in situ hybridization)
Also found in: Dictionary, Thesaurus, Medical, Financial, Acronyms, Wikipedia.

fluorescent in situ hybridization

[flə¦res·ənt in¦sit‚chü ‚hī·brə·də′zā·shən]
(genetics)
A technique in which a deoxyribonucleic acid (DNA) probe is labeled with a fluorescent dye (that can be visualized under a fluorescent microscope) and then hybridized with target DNA, usually chromosome preparations on a microscopic slide. It is used to precisely map genes to a specific region of a chromosome in prepared karyotype, or can enumerate chromosomes, or can detect chromosomal deletions, translocations, or gene amplifications in cancer cells. Abbreviated FISH.
References in periodicals archive ?
- Fluorescence In Situ Hybridization (FISH) (Technique) Global Competitor Market Share Positioning for 2019 & 2025
Assessment of methods for tissue-based detection of the HER-2/neu alteration in human breast cancer: a direct comparison of fluorescence in situ hybridization and immunohistochemistry.
Brainard et al., "Multitarget fluorescence in situ hybridization assay detects transitional cell carcinoma in the majority of patients with bladder cancer and atypical or negative urine cytology," The Journal of Urology, vol.
Japanese scientists have developed a new plating approach: Fluorescence in situ hybridization with filter-cultivation (FISHFC), which can specifically enumerate viable microorganisms.
They used a sperm fluorescence in situ hybridization assay to measure frequencies of sperm carrying partial chromosomal duplications or deletions among benzene-exposed and unexposed workers in Tianjin, China, who participated in the China Benzene and Sperm Study.
In some more traditional hospital-based laboratories, for example, there is a need for cytotechnologists to perform tests like fluorescence in situ hybridization (FISH) that determine Her2/neu gene copy number amplification on formalin-fixed paraffin-embedded breast biopsies.
In this study, the molecular cytogenetic characterization of mitotic chromosomes of the Provence flat oyster or dwarf oyster, Ostrea stentina, was performed through Giemsa staining, chromosome measurements, in situ restriction endonuclease banding, C-banding, fluorescence in situ hybridization with major ribosomal RNA genes (ITS1), and telomeric sequence [(TTAGGG).sub.n].
Fluorescence in situ hybridization (FISH) in samples of amniotic fluid obtained by early amniocentesis
The purpose of this study is to evaluate the factors contributing to false positive 3+ results by Hercep test kit IHC and subsequently not amplified for the HER-2/neu gene by Fluorescence in situ hybridization (FISH).
The remainder of the book contains review and research articles from scholars from Europe, Asia, and South America on topics such as mixtures of microorganisms in biocontrol, fluorescence in situ hybridization in aquatic bacteria, and improving fecal coliform removal and maturation ponds.
The companies also signed a probe supply agreement granting Ikonisys rights to purchase from Abbott chromosome fluorescence in situ hybridization (FISH) probes in the fields of pre-implantation genetic diagnosis and automated detection in amniocytes of FISH signals of the most common chromosomal abnormalities.
Our objective was to produce C-banding karyotypes of Arrhenatherum accessions to compare chromosome morphology with previously C-banded Arena species (Jellen et al., 1993a,b; Jellen and Gill, 1996; Jellen and Ladizinsky, 2000) and to determine the number of NOR ribosomal DNA (45S rDNA) sites using fluorescence in situ hybridization (FISH).
Full browser ?