fluorescent in situ hybridization

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fluorescent in situ hybridization

[flə¦res·ənt in¦sit‚chü ‚hī·brə·də′zā·shən]

(genetics)

A technique in which a deoxyribonucleic acid (DNA) probe is labeled with a fluorescent dye (that can be visualized under a fluorescent microscope) and then hybridized with target DNA, usually chromosome preparations on a microscopic slide. It is used to precisely map genes to a specific region of a chromosome in prepared karyotype, or can enumerate chromosomes, or can detect chromosomal deletions, translocations, or gene amplifications in cancer cells. Abbreviated FISH.

References in periodicals archive ?

Human epidermal growth factor receptor 2 (HER2) test results distribution applying 2007, 2013, and 2018 American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guidelines on a series of 880 patients with invasive breast cancer selected for in situ hybridization analysis performed with dual-probe US Food and Drug Administration--approved fluorescent in situ hybridization assay (PathVysion, Abbott, Abbott Park, IL).

Implementation of the 2018 Human Epidermal Growth Factor Receptor 2 Guideline by American Society of Clinical Oncology/College of American Pathologists Will Reduce False-Positive Tests

Rapid diagnostic of bacterial meningitis by real-time PCR and fluorescent in situ hybridization. J Clin Microbiol 2005; 43: 3390-3397.

Hibridacion in situ para la deteccion de Streptococcus agalactiae en tejidos de tilapia (Oreochromis sp.)

(6,7) Diagnosis is confirmed by fluorescent in situ hybridization (FISH) examination with a chromosome 22 library.

Partial tetrasomy of chromosome 22: genetic and surgical implications for otolaryngologists

The ThermoBrite programmable temperature controlled slide processing system automates the denaturation and hybridization steps in slide-based fluorescent in situ hybridization (F)ISH assays, and provides walk-away convenience for clinical and research personnel.

Slide processing system

Employing fluorescent in situ hybridization (FISH) or [fluorescent genomic in situ hybridization (GISH)], we studied the chromosomal composition of the seed-derived disomic plants.

Seedset on Synthetic Haploids of Durum Wheat: Cytological and Molecular Investigations

Applications include use in in situ hybridization, fluorescent in situ hybridization and other solid-phase assays where strong signal detection and sensitivity are desired."

BioGenex awarded United States patent on the synthesis of branched oligomers for nucleic acid labeling and amplification

reagents for research using fluorescent in situ hybridization (fish) 1 name, 35 000 byn2.

Reagents For Research Using Fluorescent In Situ Hybridization (fish)

(6.) Press MF, Sauter G, Buyse M, et al: HER2 gene amplification testing by fluorescent in situ hybridization (FISH): Comparison of the ASCO-College of American Pathologists guidelines with FISH scores used for enrollment in Breast Cancer International Research Group clinical trials.

Human Epidermal Growth Factor Receptor 2 Testing by Fluorescent In Situ Hybridization: Positive or Negative? American Society of Clinical Oncology/ College of American Pathologists Guidelines 2007, 2013, and 2018

The company's products and services are based on its proprietary RNAscope technology, the first automated multiplex chromogenic and fluorescent in situ hybridization platform capable of detecting and quantifying RNA biomarkers in situ at single-molecule sensitivity.

US CDC Research Shows Replication of Zika Virus in Brains of Infants with Microcephaly and Placentas of Women with Pregnancy Losses

Fluorescent in situ hybridization assay using peptide nucleic acid probes for differentiation between tuberculous and nontuberculous mycobacterium species in smears of mycobacterium cultures.

Peptide nucleic acid-based in situ hybridization assay for detection of parvovirus B19 nucleic acids