fluorescent in situ hybridization


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fluorescent in situ hybridization

[flə¦res·ənt in¦sit‚chü ‚hī·brə·də′zā·shən]
(genetics)
A technique in which a deoxyribonucleic acid (DNA) probe is labeled with a fluorescent dye (that can be visualized under a fluorescent microscope) and then hybridized with target DNA, usually chromosome preparations on a microscopic slide. It is used to precisely map genes to a specific region of a chromosome in prepared karyotype, or can enumerate chromosomes, or can detect chromosomal deletions, translocations, or gene amplifications in cancer cells. Abbreviated FISH.
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Human epidermal growth factor receptor 2 (HER2) test results distribution applying 2007, 2013, and 2018 American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guidelines on a series of 880 patients with invasive breast cancer selected for in situ hybridization analysis performed with dual-probe US Food and Drug Administration--approved fluorescent in situ hybridization assay (PathVysion, Abbott, Abbott Park, IL).
Rapid diagnostic of bacterial meningitis by real-time PCR and fluorescent in situ hybridization. J Clin Microbiol 2005; 43: 3390-3397.
(6,7) Diagnosis is confirmed by fluorescent in situ hybridization (FISH) examination with a chromosome 22 library.
The ThermoBrite programmable temperature controlled slide processing system automates the denaturation and hybridization steps in slide-based fluorescent in situ hybridization (F)ISH assays, and provides walk-away convenience for clinical and research personnel.
Employing fluorescent in situ hybridization (FISH) or [fluorescent genomic in situ hybridization (GISH)], we studied the chromosomal composition of the seed-derived disomic plants.
Applications include use in in situ hybridization, fluorescent in situ hybridization and other solid-phase assays where strong signal detection and sensitivity are desired."
reagents for research using fluorescent in situ hybridization (fish) 1 name, 35 000 byn2.
(6.) Press MF, Sauter G, Buyse M, et al: HER2 gene amplification testing by fluorescent in situ hybridization (FISH): Comparison of the ASCO-College of American Pathologists guidelines with FISH scores used for enrollment in Breast Cancer International Research Group clinical trials.
The company's products and services are based on its proprietary RNAscope technology, the first automated multiplex chromogenic and fluorescent in situ hybridization platform capable of detecting and quantifying RNA biomarkers in situ at single-molecule sensitivity.
Fluorescent in situ hybridization assay using peptide nucleic acid probes for differentiation between tuberculous and nontuberculous mycobacterium species in smears of mycobacterium cultures.
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