Modern aspects of
fluorimetry as applied to clinical chemistry.
The recombinant plasmids were purified using E.Z.N.A Plasmid Mini Kit (Omega Bio-tek), and quantified by
fluorimetry using the Qubit[R] Quantification System (Invitrogen Corp.).
Differential Scanning
Fluorimetry (DSF)/Fluorescent Thermal Shift Assay.
[30] used
fluorimetry to confirm covalent bond formation onto polymeric nanoparticle surface.
Harmer, "Determination of protein-ligand interactions using differential scanning
fluorimetry," Journal of Visualized Experiments, no.
Differential scanning
fluorimetry (DSF) revealed that C70S and C287S have little effect on SULT1A1 thermal stabilities.
A 10 mm optical length quartz cuvette for
fluorimetry was used in these measurements, which were done in a front face configuration mode.
A newborn screening study using digital microfluidics
fluorimetry with 4-methylumbelliferyla-glucoside to measure GAA activity carried out in the Missouri state newborn screening laboratory yielded 84 screen positives out of 175000 newborns (8), a screen positive rate that was 2.5-fold higher than the value obtained by MS/MS in Washington and New York.
The release of nile red was monitored using
fluorimetry (JASCO, FP-8200, excitation at 550 nm and emission at 630 nm).
Permeability to the Lucifer yellow dye (LY, Sigma-Aldrich L0259) was next measured over 60 min using
fluorimetry, as described [32].
Notice that we did not detect significant changes when mean PI uptake was measured by
fluorimetry in the cell cultures incubated with MG at 1 mM, as shown in Figure 2(c).