Fluorochrome


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Fluorochrome

 

a substance used in fluorescence, or luminescence, microscopy to study objects that do not possess a natural capacity to fluoresce. When fluorochromes are introduced into an organism, they are adsorbed by the cells, which consequently acquire the capacity to fluoresce. Fluorochromes may be dyes (auramine, coryphosphin), pigments and their derivatives (chlorophyll, porphyrin), or alkaloids (berberine). Fluorescence in microscopic objects stained with fluorochromes is brought about by ultraviolet, violet, or blue light. Fluorescence microscopy with the use of fluorochromes brings out structural details more clearly than does ordinary staining, especially in the case of biological specimens. Because the fluorescence microscopy method is highly sensitive, the fluorochrome concentration can be very low and consequently living organisms can be observed and their metabolic processes studied.

REFERENCES

Levshin, V. L. Fotoliuminestsentsiia zhidkikh i tverdykh veshchestv. Moscow-Leningrad, 1951.
Zelenin, A. V. Liuminestsentnaia tsitokhimiia nukleinovykh kislot. Moscow, 1967.
References in periodicals archive ?
Abbreviations: HER2, human epidermal growth factor receptor type 2; MEF, molecules of equivalent fluorochrome.
where G is Green's function solution to the diffusion equation and f is the fluorescent yield of fluorochrome. [G.sub.em]([r.sub.d],r) denotes the photon density for emission at the position of detector [r.sub.d] when a point source is located at position r.
To determine the density and biomass, semi-permanent glass slides were prepared by filtering samples through a black filter (Nucleopore/Watchman 0.8 pm) and stained with DAPI (fluorochrome 4'-6-diamidino-2-phenylindole at 0.001%) for 15 minutes in the dark (PORTER; FEIG, 1980).
During the oxidative burst, the fluorochrome DHR 123 reacts specifically with the cellular [H.sub.2][O.sub.2] oxidising the dye to rhodamine (1).
To investigate the periodicity of formation of striae and the increment of shell deposited under the different environmental conditions, each scallop was marked individually using the fluorochrome dye calcein.
Briefly, the blastocysts were treated with a permeabilising solution containing the ionic detergent Triton X-100 and the fluorochrome propidium iodide (cat.NO.P-4170, Sigma).
These are based on non-fluorescent substrates that release free fluorochrome into the cytoplasm when enzymatically cleaved.
On day 5, the embryos were fixed in 4% PFA for 30 min, permeabilized with 0.2% Triton X-100 for 10 min and stained with 5 [micro]g/ml fluorochrome 4,6-diamidino-2-phenylindole (DAPI, Roche 236276, UK) in PBS for 20 min.
For the fluorochrome labeling, 10[micro]L fluorescein-5- isothiocyanate (FITC) labeled goat anti-mouse IgM ([micro]) antibody (Sigma, St.