Fluorochrome


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Fluorochrome

 

a substance used in fluorescence, or luminescence, microscopy to study objects that do not possess a natural capacity to fluoresce. When fluorochromes are introduced into an organism, they are adsorbed by the cells, which consequently acquire the capacity to fluoresce. Fluorochromes may be dyes (auramine, coryphosphin), pigments and their derivatives (chlorophyll, porphyrin), or alkaloids (berberine). Fluorescence in microscopic objects stained with fluorochromes is brought about by ultraviolet, violet, or blue light. Fluorescence microscopy with the use of fluorochromes brings out structural details more clearly than does ordinary staining, especially in the case of biological specimens. Because the fluorescence microscopy method is highly sensitive, the fluorochrome concentration can be very low and consequently living organisms can be observed and their metabolic processes studied.

REFERENCES

Levshin, V. L. Fotoliuminestsentsiia zhidkikh i tverdykh veshchestv. Moscow-Leningrad, 1951.
Zelenin, A. V. Liuminestsentnaia tsitokhimiia nukleinovykh kislot. Moscow, 1967.
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References in periodicals archive ?
Abbreviations: HER2, human epidermal growth factor receptor type 2; MEF, molecules of equivalent fluorochrome.
Quantitative Assessment of Immunohistochemistry Laboratory Performance by Measuring Analytic Response Curves and Limits of Detection
where G is Green's function solution to the diffusion equation and f is the fluorescent yield of fluorochrome. [G.sub.em]([r.sub.d],r) denotes the photon density for emission at the position of detector [r.sub.d] when a point source is located at position r.
Image Restoration for Fluorescence Planar Imaging with Diffusion Model
* The use of beads hard-dyed with fluorochrome surrogates rather then the fluorochromes themselves; and
A new method for quantitative standardization of flow cytometry instruments: flow cytometry is increasingly being used in the biopharma and healthcare sectors
Fluorochromes were purchased from Invitrogen (Carlsbad, CA).
Identifying the cellular mechanisms of symbiont-induced epithelial morphogenesis in the squid-vibrio association
To determine the density and biomass, semi-permanent glass slides were prepared by filtering samples through a black filter (Nucleopore/Watchman 0.8 pm) and stained with DAPI (fluorochrome 4'-6-diamidino-2-phenylindole at 0.001%) for 15 minutes in the dark (PORTER; FEIG, 1980).
Flagellate protist abundance in phytotelmata of Aechmea distichantha Lem. (Bromeliaceae) in the upper Parana river basin/Abundancia de protozoarios flagelados em fitotelmata de Aechmea distichantha Lem. (Bromeliaceae) na bacia do alto rio Parana
During the oxidative burst, the fluorochrome DHR 123 reacts specifically with the cellular [H.sub.2][O.sub.2] oxidising the dye to rhodamine (1).
Comparison of two methods for the diagnosis of chronic granulomatous disease--neutrophil oxidative burst measured by the nitroblue tetrazolium slide test versus the dihydrorhodamine 123 flow cytometric assay
To investigate the periodicity of formation of striae and the increment of shell deposited under the different environmental conditions, each scallop was marked individually using the fluorochrome dye calcein.
Nondaily deposition of striae in the bay scallop argopecten irradians (Concentricus) in the laboratory
Briefly, the blastocysts were treated with a permeabilising solution containing the ionic detergent Triton X-100 and the fluorochrome propidium iodide (cat.NO.P-4170, Sigma).
Effect of vitrification on number of inner cell mass in mouse blastocysts in conventional straw, closed pulled straw, open pulled straw and cryoloop carriers
These are based on non-fluorescent substrates that release free fluorochrome into the cytoplasm when enzymatically cleaved.
Validation of rapid microbiological enumeration methods: contamination control strategies to reduce risk, optimize yields and improve quality
Validation of annulus formation in otoliths of a temperate population of adult African sharptooth catfish Clarias gariepinus using fluorochrome marking of wild fish.
Comparison of age estimates from otoliths, vertebrae, and pectoral spines in African sharptooth catfish, Clarias gariepinus (Burchell)/Otoliitide, selgroolulide ja rinnauimekiirte alusel maaratud vanuse vordlus Aafrika sagal Clarias gariepinus (Burchell)
On day 5, the embryos were fixed in 4% PFA for 30 min, permeabilized with 0.2% Triton X-100 for 10 min and stained with 5 [micro]g/ml fluorochrome 4,6-diamidino-2-phenylindole (DAPI, Roche 236276, UK) in PBS for 20 min.
Down-regulation of HLA-G attenuates cleavage rate in human triploid embryos
For the fluorochrome labeling, 10[micro]L fluorescein-5- isothiocyanate (FITC) labeled goat anti-mouse IgM ([micro]) antibody (Sigma, St.
Presence of a D8/17 B lymphocyte marker and HLA-DR subgroups in patients with rheumatic heart disease/Romatizmal kalp hastalarinda bir lenfosit belirteci olan D8/17B'nin ve HLA-DR alt gruplarinin varligi

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