The present work is a follow-up investigation on the practical use of Molecules of Equivalent Soluble Fluorophore
values, MESF, defined and published earlier (6).
Our observations may also prove important in using GFP and related compounds in the application of FRET (fluorescence resonance energy transfer) and other measurements of molecular distances and orientations, because the interpretation of these measurements relies on the knowledge, or assumptions, of the orientation-dependent polarizability of the fluorophores
For example, specific DNA sequences are detected through hybridization to complementary strands that carry the coding information (different fluorophore
labels), whereas specific proteins are detected by using antibodies that target different epitopes on the surface of the protein target.
In such cases the molar extinction and quantum yield of the fluorophore
is the same for the analyte and the reference material and the fluorescence radiance from both is proportional to the relative number of fluorophores
Past research in the Bellarmine Laboratory used the predictable spectroscopic character of natural fluorophore
mixtures that are observed on serial dilution to characterize petroleum-contaminated samples.
That question starts with whether UV light can penetrate ice to reach the water below where it's available to "excite" the fluorophore
in a lure's coating.
The skin chromophore maps are calculated from the four spectral images, and the perfusion map and fluorophore
distribution map--from the video-images taken at green and ultraviolet illumination of the skin, respectively.
The lack of fluorophore
leakage from FF beads during magnetic separation indicates that all fluorophores
are retained firmly inside the polymer of the beads.
3), the relative QY of QD605 modified with CD14 was measured using fluorescein as the reference fluorophore
with a known quantum yield of 0.
o] (ns) is the lifetime of the fluorophore
in the absence of quencher.
Due to the release of the fluorophore
, initially linked to the protein, the fluorescent signal is enhanced after protein cleavage.
The principle of operation is to trap an oxygen-sensitive fluorophore
or pH indicator dye in a sol-gel host matrix that can be applied to the tip of a fibre, an adhesive membrane such as a patch or a flat substrate such as a cuvette.