Here, we demonstrate the features and sources of AF in FFPE bone marrow tissue, and we present a simple, efficient method to eliminate AF without decreasing the intensity of fluorophores
or affecting the combination of fluorescent markers with tissues.
The presented fluorescent Cys probe (1) was obtained by incorporating the DNBS functional group (a well-known recognition moiety for the biothiols) onto the coumarin-based fluorophore
. Probe 1 is nonfluorescent due to the quenching effect of DNBS unit via the electron-transfer process.
To completely avoid the potential of fluorophore
interactions, different fluorophores
could be used with nonoverlapping spectra.
Past research in the Bellarmine Laboratory used the predictable spectroscopic character of natural fluorophore
mixtures that are observed on serial dilution to characterize petroleum-contaminated samples.
The measurements of the fluorescence intensity from dilutions of the reference fluorophore
solutions were always performed from the low to high concentrations.
Molecules in substances called fluorophores
are responsible for this action.
The skin chromophore maps are calculated from the four spectral images, and the perfusion map and fluorophore
distribution map--from the video-images taken at green and ultraviolet illumination of the skin, respectively.
Examples comprise transferring the molecules to a transient dark state , optical transfers between two distinguishable fluorophore
isomers (e.g., 'photoswitching' fluorophores
), and others .
Nagano and Yoshimura developed NO probes containing organic fluorophores
, in which the fluorescence of the organic probe was turn-on upon oxidation by dinitrogen trioxide ([N.sub.2][O.sub.3]) formed from the NO oxidation in the presence of oxygen .
Gold thiolate crystals have a polymeric structure with sulphur atoms bridging the gold atoms and can generate a fluorescence depending on the concentration of S-Au-Au fluorophores
groups present in the system.
If the fluorescence emissions originating from the fluorophore
or the metal surface are indistinguishable, therefore, the emitting complex (dye/metal surface) could more accurately be treated as an entity with the proposed name plasmophore .
The ability to visualize differently fluorescent tumors simultaneously resulted in the identification of an optimal fluorophore
for fluorescence laparoscopy .