SDF-1, a chemokine with a
G protein coupled receptor, or BDNF, act as chemoattractants for cerebellar granule cells, with SDF-1 action being selectively inhibited by soluble EphB receptor.
The vector contains a first segment comprising at least a fragment of the extreme amino-terminal coding sequence of a yeast
G protein coupled receptor. A second segment is positioned downstream from the first segment (and in correct reading frame therewith), with the second segment comprising a DNA sequence encoding a heterologous
G protein coupled receptor.