A three-dimensional structure of GATA3
was built by Swiss-model (http://swissmodel.expasy.org/workspace/) and then visualized by Swiss-PdbViewer (version 4.1, http://spdbv.vital-it.ch/).
For quantification of Foxp3, Gata3
, Rorc, Tnfa, Tbx21, Ccr2, and CCr5, the gene expression assays Mm00475162_m1, Mm00484683_m1, Mm01261022, Mm00443258_m1, Mm00450960, Mm00438270, and Mm01216171 (Applied Biosystems, Foster City, DA, USA) were used, respectively.
These features were suspicious for a high-grade subtype of urothelial carcinoma, and thus immunohistochemistry was performed with a Ventana Benchmark Ultra using antibodies to bcl-2 (mouse monoclonal, ready-to-use, Ventana), keratin 20 (rabbit monoclonal, ready-to-use, Ventana), desmin (mouse monoclonal, ready-to-use, Ventana), p16 (mouse monoclonal, ready-to-use, Ventana), p40 (mouse monoclonal, ready-to-use, Ventana), GATA3
(mouse monoclonal, ready-to-use, Cell Marque), p53 (mouse monoclonal, ready-to-use, Ventana), and OSCAR pankeratin (mouse monoclonal, ready-to-use, Cell Marque).
Each Th cell subset expresses a unique set of transcription factors and produces hallmark cytokines , with Th2 cells being controlled by the master transcription factor, GATA3
. Differentiated Th2 cells secrete IL-4, IL-5, and IL-13, which are important in inflammatory asthma and atopy, and constitute a defense against extracellular pathogens during inflammation.
Quantitative realtime polymerase chain reaction (qPCR) of interleukin (IL)-4, IL-10, IL-13, interferon (IFN)-[gamma], tumor necrosis factor (TNF)-[alpha], IL-2, T-bet, STAT1, and GATA3
was performed on equal amounts of cDNA using GoTaq qPCR Master Mix (Promega).
Immunohistochemical studies were carried out on the cell block and core biopsy sections using antibodies TTF-1 (1:200; Leica), thyroglobulin (1:1000; Dako), ER (1:50; Dako), PR (premade; Leica), GATA3
(1:200; Cell Marque), GCDFP-15 (1:500; Leica), CD56 (Premade; Leica), synaptophysin (1:200; Dako), chromogranin (1:200; Cell Marque), pAx8 (1:100; Cell Marque), villin (1:100; Leica), CK 7 (1:400; Dako) and CK20 (1:200; Novocastra).
Early phase overexpression of GATA2 and GATA3
are negatively regulate adipogenesis through downregulation of PPAR[gamma] and C/EBP[alpha] and is supported by many studies including Berberine (Hu and Davies, 2009; Tong et al., 2005).
Andrographolide also increased GATA3
mRNA expression but decreased T-bet and ROR[gamma]t mRNA expressions .
TORONTO, Ont., August 25, 2013 -- Stem cell scientists here have discovered the gene GATA3
has a role in how blood stem cells renew themselves, a finding that advances the quest to expand these cells in the lab for clinical use in bone marrow transplantation, a procedure that saves thousands of lives every year.
Un estudio reciente de asociacion del genoma completo de LH ha confirmado la fuerte relacion entre la region del HLA y el riesgo de LH e identificaron tres nuevos loci de susceptibilidad en 2p16.1 [rs1432295, gen REL, odds ratio (OR) = 1,22], 8q24.21 [rs2019960, gen PVT1, OR = 1,33] y 10p14 [rs501764, GATA3
, OR = 1,25] (71).
Mutations in GATA3
, a dual zinc-finger transcription factor, cause the autosomal dominant hypoparathyroidism, deafness, renal dysplasia (HDR) syndrome, and down-regulation of GATA3
results in clear cell renal cell carcinoma.
UCSF scientists describe for the first time how the protein, known as GATA3
- which is abnormal or absent in many cases of human breast cancer - normally acts downstream in biochemical pathways to prevent the distant spread of cancer, an event called metastasis.