Specificity and sensitivity of a gene dose increase as neoplastic marker can be expressed as the fraction of pathologically determined tumor tissues of the group where measured gene dose exceeds a given threshold.
Gene dose results for each marker were also correlated as function of the phenotype by means of the Wilcoxon signed rank test.
Finally, a Spearman analysis was used to find any correlation of the gene dose with the age of the patients.
Genotypes traditionally Measured associated with Representative functional Semiquantitative EM (a) phenotype genotype gene dose gene dose Active/Active *1/*1 Reference 2.
This concept of semiquantitative gene dosing was validated by demonstrating that semiquantitative gene doses of 0.
To allow comparison with published data, we assigned a semiquantitative gene dose (SGD) of 1 for functional alleles, 0 for completely dysfunctional null alleles (e.
The combined influence of CYP2C19 gene dose and CYP2D6 SGD is shown in Table 4.
Two studies have quantitatively examined the correlation between CYP2D6 gene dose (number of functional genes) and NT concentrations (35, 36).
The gene dose was then compared with reported values for the cell lines (12, 28, 29).
Although there was a decrease in the apparent gene dose with the single-point estimate, amplification was still apparent in the abnormal (T-47D and SK-BR-3) cell lines.
For the 10 nonpathological samples tested with a single-point estimate, the mean gene dose and SD was 0.
Normal ized gene doses
N were then determined for each sample in three independent assays, and the corresponding means were calculated.