Malaria epidemiology, glucose 6-phosphate
dehydrogenase deficiency and human settlement in teh Vanuatu Archipelago.
Altikat, "Purification of glucose 6-phosphate
dehydrogenase from buffalo (Bubalus bubalis) erythrocytes and investigation of some kinetic properties," Protein Expression and Purification, vol.
dehydrogenase (G6PD) deficiency is a posible risk factor for the development of preeclampsia.
The activities of hexokinase, glucose 6-phosphatase, fructose 1,6-bisphosphatase, glucose 6-phosphate
dehydrogenase, pyruvate kinase and glycogen were assayed by the methods of Brandstrup et al.
The product, known as glucose 6-phosphate
, is immediately transported across the outer membrane of mitochondria and is further processed within this organelle (see Chart 3).
Thus glucose continues to be metabolized to glucose 6-phosphate
, which is further metabolized to other phosphorylated metabolites of glucose, all of which accumulate in the cells.
Studies to rule out underlying immunodeficiency showed no evidence of glucose 6-phosphate
dehydrogenase (G6PD) deficiency or HIV infection.
Spectrophotometric assays were used to determine the activity of glucose 6-phosphate
dehydrogenase (G6PDH) , isocitrate dehydrogenase (ICDH) , malate dehydrogenase (MDH) , and malic enzyme (ME) .
For assessment of the presence of the inactivator(s), the purified nNOS (80 [micro]g/ml) was added to a 'first reaction mixture' of 40 mM potassium phosphate, pH 7.4, containing 0.2 mM Ca[Cl.sub.2], 500 unit/ml superoxide dismutase, 100 units/ml catalase, 80 [micro]g/ml calmodulin, 100 [micro]M [BH.sub.4], 1 mM dithiothreitol, the desired concentration of extract, and an NADPH-generating system composed of 0.4 mM [NADP.sup.+], 10 mM glucose 6-phosphate
, and 1 unit of glucose 6-phosphate
dehydrogenase/ml, expressed as final concentrations, in a total volume of 180 [micro]l.
An elevation of aminotransferases in the range of viral hepatitis was common, but hyperbilirubinemia is exceptional and usually associated with alcoholism, co-infection, or glucose 6-phosphate
The next irreversible reaction is the conversion of fructose 1,6-bisphosphate to glucose 6-phosphate
by the enzyme Fructose 1,6-bisphosphatase (FBP1).