The acyl glucuronide
of MPA (AcMPAG) and the carboxybutoxy ether of MPA (MPAC) were kind gifts of Hoffmann-La Roche AG (Basel, Switzerland).
Commercial calibrators (5[beta]-pregnane-3[alpha],20[alpha]-diol glucuronide
In continuation of studies of steroid profiles in diseases related to steroid enzymes (7, 8), we here demonstrate a sensitive and precise HPLC-tandem MS method for quantification of testosterone and DHT glucuronides
by use of a three-column, two-switching valve system and the presence of the indicative biomarker in urine samples from BPH patients.
A similar observation was reported for acyl glucuronides
based on a comparison of their disappearance in buffer and in solutions of buffered serum albumin (27, 28).
STABILITY OF STEROID GLUCURONIDES
IN DRIED BLOOD SPOTS
revised: is the glucuronidation process a toxification as well as a detoxification?
On-line deconjugation of glucuronides
using an immobilized enzyme reactor based upon [beta]-glucuronidase.
It has been reported that glucuronide
conjugates can be determined directly by LC/MS/ MS.
Probenecid, uricosuric drug in the treatment of gout, interferes with renal excretion of steroid glucuronides
. Inhibitors of 5[alpha] reductase, the class of pharmacological agents in the treatment of benign prostatic hyperplasia, alter metabolism of anabolic steroids reducing excretion of dihydrotestosterone and androsterone.
For BPF and BPAF, we used wider ranges and varied the EHR rates of unconjugated substances and glucuronides
in the uncertainty loops (for BPA and BPS, we varied them only in the variability loops).
Interestingly, serum levels of some of the bile acid glucuronides
, such as CDCA-3G, CA-24G, and LCA-3G, were significantly reduced after biliary stenting, while some others, like CDCA-24G, LCA-24G, and HDCA-6G, were significantly increased.
Increasing the number of glucuronides
at position 7 of the A ring (compound 6) in the skeleton of compound 10 increased its inhibitory activities on protein glycation.