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[36], gellan gum is composed of tetrasaccharide (1.3-[beta]-D-glucose, 1.4-[beta]-D-glucuronic acid, 1.4-[beta]-D-glucose, and 1,4-[alpha]-L-rhamnose) repeating units with a molar ratio of 2: 1: 1 containing about 1.5% acyl groups as acetyl and glycerate molecules per unit (Figure 1).
In terrestrial plants, glycolate is metabolized in peroxisomes and mitochondria (where [CO.sub.2] is released), producing glycerate. Glycerate is taken up by chloroplasts where it and PGA participate in regeneration of the [RuP.sub.2] substrate used by rubisco.
Other acids were also included in preliminary experiments: pyroglutamate, glycerate, p-hydroxyphenyllactate, sebacate, citrate, adipate, succinate, glutarate, and oxalate.
[1] on improving the extraction and therefore the quantification of urinary glycerate and glycolate.
Currently this is accomplished by determination of AGT activity in liver biopsy specimens [6, 7] and measurement of glycolate and glycerate excretion.
A preliminary experiment showed that glycerate and glycolate were stable in acidified urine for up to 3 months.
Quantification of oxalate, glycolate, and glycerate was based on the detector response to a known amount of each compound and corrected for recovery of the internal calibrator.
To determine the optimal number of extractions, 250 [micro]g each of oxalate, glycerate, glycolate, and ethylphosphonate were added to an aliquot of normal urine (containing 500 mg creatinine) that was extracted three times with ether and then repeatedly extracted with THF.
Glycerate excretion in this healthy population was dependent on age (Fig.
The new extraction protocol was applied to specimens from 16 PH patients seen at the Mayo Clinic Division of Nephrology to assess the ability of improved glycolate/ glycerate extraction to discriminate between PH I and PH II.
The probable production enhancement might be that the consortia of amylases and lipases act in a synergistic manner in amylolytic degradation of starch into glucose monomers which upon further splitting yield glycerates that triggers lipid metabolism within thallus.Conclusively we are of the opinion that A.