The samples and the reagents (Pipes buffer, 4 - chlorophenol, Magnesium ion, ATP, lipase, peroxidase, glycerol kinase
, 4-amino antipyrine, glycerol 3-phosphate oxidase, detergents, preservative, and stabilizer) were mixed well using a vertex.
Several enzymes are involved in the incorporation of these substrates in the gluconeogenesis pathway : lactate dehydrogenase (LDH) for lactate; glycerol kinase
(GK) and glycerol-3-phosphate dehydrogenase (GPD) for glycerol; and acyl-CoA synthetase short-chain family member 3 (ACSS3), propionyl-CoA carboxylase (PCC), methylmalonyl-CoA epimerase (MCEE), and methylmalonyl-CoA mutase (MUT) for propionate.
Chromosomal microdeletions and duplications are one of the major causes of congenital malformations and developmental delay.Lately by the effect of array-CGH,many new syndromes are defined in patients with congenital malformations and developmental delay.Xp21 contiguous gene deletion syndrome is one of the rare microdeletion syndromes.This syndrome is characterized by developmental delay,congenital adrenal hypoplasia, DuchenneMuscularDystrophy (DMD) and glycerol kinase
For the detection of glycerol kinase
(GK) and glucokinase (GCK), similar concentrations of total protein were resolved in an SDS-PAGE system at 12% acrylamide concentration.
The seven genes are: arc (carbamate kinase), aroE (shikimate dehydrogenase), glpF (glycerol kinase
), gmk (guanylate kinase), pta (phosphate acetyl transferase), tpi (triosephosphateisomerase), yqiL (acetyl coenzyme A acetyl transferase).
Complex glycerol kinase
deficiency (CGKD) is a contiguous gene deletion syndrome which is inherited as an X-linked trait.
Metabolism of glycerol to fatty acid has been reported in rat liver microsomes and is a result of the metabolism of triglycerides by adipose tissue and other tissues that possess the enzyme that activates glycerol, namely glycerol kinase
. High glycerol kinase
levels are found in liver and brown tissues.
NR0B1 (DAX1) deletion may occur in isolation or as part of a contiguous gene deletion syndrome at Xp21 including the dystrophin gene (DMD) and the glycerol kinase
Cloning, heterologous expression and kinetic analysis of glycerol kinase
(TbGLKl) from Trypanosoma brucei.
After that, the glycerin is converted into glycerol-3-phosphate by glycerol kinase
, and then it is oxidized to dihydroxyacetone phosphate on the outer face of the inner mitochondrial membrane and goes to the glycolytic pathway for energy production (Robergs & Griffin, 1998).
The assay employs a coupled enzymatic reaction system in 96-well plates whereby plasma glycerol content is converted by glycerol kinase
. The enzymatic reaction produces glycerol-3-phosphate (G3P) in the presence of adenosine-5'-diphosphate (ADP).
Glycerol is obtained as glycerol-3-phosphate (G3P) from glycolysis, glyceroneogenesis, and phosphorylation of glycerol by glycerol kinase