The reference text offers cell biologists discussion of topics such as the architecture of the mammalian Golgi, Golgi glycosylation and human inherited diseases, a critical assessment of models for Golgi traffic, entry and exit mechanisms at the cis-face of the Golgi complex, COPI budding within the Golgi stack
, Golgin coiled-coil proteins, organization of SNAREs in the Golgi stack
, protein retention mechanisms, Golgi transport proteins structure, Golgi and related vesicle proteomics, entry at the trans-face of the Golgi, the signaling network, Golgi positioning, Golgi development, Golgi biogenesis, and evolution and diversity of the Golgi.
EM micrograph of follicular cell of goat thyroid (3 years) showing Golgi stacks
(G) close to the nucleus (N).
Multi-membrane spanning Gts (cellulose and callose synthases) are typically associated with the plasma membrane, while both Gts classes type II and multimembrane spanning have been found in Golgi stacks where they are likely involved in the biosynthesis of complex [beta]-glycan polymers [7-9].
The chimera was localized in the Golgi stacks in a functional organization since an increase in mannan synthesis was quantified in transformed protoplasts.
Treatment with Brefeldin A, a lipophilic fungal toxin known to induce disassembly of the Golgi stacks among other effects , determined the aggregation of the punctate structures (Figures 1(b) and 1(c)) and, during time, the redistribution of the fluorescence into the tubular and cortical network (Figure 1(d)).
The results indicate that the catalytic site of AtCslA2 faces the lumen of the Golgi stacks, according to bioinformatic predictions.
Their cytoplasm encloses one or two juxtanuclear Golgi stacks, a well-developed RER, a few lysosomal bodies, many mitochondria, and numerous clear vesicles of various sizes.
On some micrographs, the Golgi stacks show cisternae ending with spheroidal distentions that enclose a fuzzy materi al of low electron density (Fig.
Type 3 cells possess distended RER cisternae and Golgi stacks ending in spheroidal distentions.