Gram's Method

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Gram’s Method

 

a method for the differential staining of bacteria, proposed in 1884 by the Danish physician H. C. Gram.

In Gram’s method the bacteria are stained with basic dyes, including crystal or methyl violet, which are then fixed in an iodine solution. After rinsing the dyed preparation in alcohol some types of bacteria remain thoroughly stained (the so-called gram-positive bacteria), and others (gram-negative) are decolorized. Such a division is of great importance in the classification of bacteria and in the microbiological diagnosis of infectious diseases.

References in periodicals archive ?
KOH preparation along with Gram staining is a reliable method for detecting fungal aetiology as compared to fungal culture, as culture takes much longer incubation for most fungal etiological agents.
Diagnosis of S.aureus mastitis can be done by direct milk culture for detection of bacteria as well as some indirect test like MCMT, gram staining for detection of gram positive staphylococcus colony and leukococytosis in hematological examination (Boerlin et al., 2003; Tenhagen et al., 2009).
sibiricum as a pathogen and not a contaminant because it was the only organism isolated, and Gram staining of the exudate revealed leukocytes.
Upon gram staining, 4 isolates were identified as gram positive bacteria, while the 12 isolates shown as gram negative bacteria.
We limit this discussion to six desired features, summarized in the priority shown from the input of more than 50 high-volume gram staining labs:
1), including Gram staining, subcultivation, and biochemical identification, we assumed a referenced mean TAT of 48 h (16).
vaginalis, but for the full characterization of the smears the joint application of PCR and Gram staining is the best choice.
Application of this staining to detect Wolbachia was used during the study period and was found to be more efficient than the Gram staining (Fig.
For the time being, Gram staining and bacterial culture remain the cornerstones of bacterial meningitides diagnosis in a clinical setting.
Gram staining was done to differentiate bacteria largely into two groups, Gram-positive and Gram-negative.
On the basis of findings of culture, Gram staining and WBC counts in CSF, patients were divided into 3 groups.
aureus isolates were confirmed by mannitol fermentation and gram staining. A total of 134 S.