Reliably high deuterium toxicity was detected in the most deuterated growth medium
(D/H 500.000 ppm).
For the MTT assay, after 3 days of cell culture, the growth medium
was removed, centrifuged at 500 x g for 10 min to remove cellular debris, aliquoted, and frozen at -80[degrees]C, for later use in the tube formation assay (see below).
Mean density of the cells grown in each volume of the growth medium
was significantly different from each other (p less than 0.05).
Primary Human vSMCs, all between passage 2 and passage 6, were seeded T75 cell culture flasks with 10 mL of Basal growth medium
as previously established.
: Casein sucrose yeast (CSY) broth, Tryptose soya (TS) broth, Brain heart infusion (BHI) broth and nutrient broth were used to study the affect of medium on the growth of P.
The size of the orbit will affect how the bacterial cells circulate and how the growth medium
The day before transfection, 5x105 cells were seeded per 60 mm dish in 5 ml appropriate growth medium
containing serum and antibiotics.
max was suppressed most strongly in each growth medium
The growth medium
on the Petri dishes is then replaced every other day.
However, the decrease in counts depended upon the growth medium
and the conditions used for enumeration, with a non-selective medium giving the highest count under ROS-neutralized conditions.
Trypsin activity was inhibited by adding growth medium
and cells were centrifuged at 1000 rpm for 5 rain.
Discher of the University of Pennsylvania in Philadelphia and his colleagues had a hunch that a stem cell's fate might depend on the texture of the growth medium
that researchers use to raise the cells in the lab.