Hb Average RBC Lifespan in Days (n) Hb A
120 Hb S 93 (3) (20) Hb E No Data Hb C 87 (6) (18) Hb D 115 (3) (19) Table 2.
1c] can be separated from Hb A
because glycation of the N-terminal valine decreases the positive charge.
Several methods, including the D-10, DiaSTAT, Dimension RxL, DS5, HA8160, and VARIANT II, showed increased scatter when samples containing Hb C or S trait were tested compared with that seen for samples homozygous for Hb A
3, lane 4), did not separate from Hb A
on electrophoresis but could be easily detected by HPLC.
HPLC and electrophoretic findings include absence or marked reduction in Hb A
with a variable Hb F concentration.
Our data for Hb AS samples with the Diamat method were not consistent with previous results (5, 6).
This variant eluted after Hb A
in both the Variant (Fig.
Samples from patients homozygous for Hb A
served as controls for detecting calibration differences between methods.
2], Hb S, Hb F, and Hb A
displayed migration times of 4.
2], Hb E, Hb A
, and Hb F was performed by the BTS program; quantitation of Hb Bart's was performed by the ATS program on the Bio-Rad VARIANT, a fully automated HPLC system that uses double wavelength detection (415 and 690 nm).
, assay of the red cell hemolysates of the specimens was carried out with an automated Diamat HPLC system (BioRad), which involved a spherical cation-exchange gel column and a 5-min step-gradient created by a single-piston pump and three phosphate buffers of increasing ionic concentration.
A Hb electrophoresis panel consisting of a screen for sickle Hb, quantification of Hb fractions (normally Hb A
, Hb [A.