Hematoxylin


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Related to Hematoxylin: eosin, hematoxylin bodies

hematoxylin

[‚hē·mə′täk·sə·lən]
(organic chemistry)
C16H14O6 A colorless, crystalline compound occurring in hematoxylon; upon oxidation, it is converted to hematein which forms deeply colored lakes with various metals; used as a stain in microscopy.

Hematoxylin

 

a dye used in microscopy for staining plant and animal tissues. Hematoxylin is extracted by ether from the colored wood of the logwood tree, which is native to Central America and the Antilles Islands. In the course of its preparation for use in microscopy, the substance is “matured,” or oxidized to hematein, which stains cell nuclei, chromosomes, and cell membranes a blue or blue-black color.

REFERENCE

Romeis, B. Mikroskopicheskaia tekhnika. Moscow, 1953. (Translated from German.)
References in periodicals archive ?
Immunohistochemical reaction (IHC) with 3,3'-diaminobenzidine (DAB) chromogen and hematoxylin counterstain.
Histopathologic examination of hematoxylin and eosin-stained liver sections revealed extensive areas of necrosis and cellular debris with associated predominately granulocytic infiltrates scattered throughout the hepatic parenchyma.
Some of the tissue sections from the heart, lungs, liver, and spleen were stained with alum hematoxylin (Harris hematoxylin) and counterstained with 90% ethanolic extract of Z.
The investigators defined microvesicular steatosis as the presence of "non-zonal contiguous patches of foamy hepatocytes with centrally placed nucleus" on hematoxylin and eosin staining under light microscopy, she added.
The illustrated chapters include fixation and processing, microtomy, frozen sections, hematoxylin and eosin, Gram stain, myobacteria, Heliobacter pylori, spirochetes, fungi, trichrome stains, reticulin, elastin stains, basement membranes, mucin stains, amyloid, and immunohistochemistry.
Two mm punch biopsies of the lateral cheeks were performed at 12 weeks in four patients and stained with hematoxylin and eosin, as well as in situ hybridization studies using an anti-sense probe for mRNA for type I collagen.
Heat-shocked para (ts) fly brains and wild type flies were sectioned and stained using Hematoxylin and Eosin.
Upon hematoxylin and eosin tissue staining, rats fed the chow diet and given repeated alcohol binges showed minimal steatosis, while rats fed the [choline-deprived] diet and given repeated whisky binges showed periportal and pericentral microvesicular steatosis.
Samples were then embedded in paraffin wax, sectioned at 5 [micro]m, mounted, and stained with hematoxylin and eosin.
Routine hematoxylin and eosin stained sections were examined.