Sex of the hosts was determined by direct inspection of gonads or presence/absence of
hemipenes. The body cavity of all specimens other than those from Minas Gerais and Sao Paulo was opened by a longitudinal incision from throat to vent, the gastrointestinal tract was slit longitudinally, and the body cavity, lungs, gall bladder, lungs, stomach, and intestinal contents were removed and examined under a stereomicroscope.
Adicionalmente, se registro el estadio de vida (adulto o juvenil) y el sexo de los individuos mediante la observacion de un abultamiento a nivel de la cloaca en los machos, que indica la presencia de
hemipenes.
One pair of light orange or pink
hemipenes is located at the base of the cloaca (Fig.
gadovi, la diferenciacion sexual solo fue posible en los adultos, y se uso como criterio la presencia de
hemipenes, forma general de la cabeza y cuerpo, asi como los patrones de color en la region ventral del cuerpo, ya que en los machos el vientre esta mas pigmentado de color negro que en las hembras (Good 1988).
Male hybrids were easily identified, once captured, by the pronounced blue coloration of the ventral surface and the broad base of the tail; sex could be confirmed by using thumb-pressure to evert
hemipenes. In contrast, female hybrids and individuals of A.
Male reptiles have paired reproductive organs called
hemipenes. Gender determination of some lizards can be done by locating the
hemipene bulge, which is just cranial to the vent.
Los testiculos de un macho de 56.10 mm de LHA, midieron 4.8 X 3.4 mm, sus tubos seminales tuvieron una longitud de 7.2 X 0.4 mm; y presentaron forma serpentinada de color cafe claro y punteado, en el extremo apical de los tubos seminales, se encuentran presentes los
hemipenes (Figura 5a-b).
Each offspring was measured for snout vent length (SVL) and total length (TL), and then weighed to the nearest 0.01 gram and its sex determined by eversion of the
hemipenes in males (Gregory & Larsen 1996).
The
hemipenes are small, simple, unicapitate, with bifurcate sulcus spermaticus; the extremity is clearly bilobed but not divided; the apical third (asurcate surface) to the half-way point (surcate surface) covered with calyculate spines; the central sector is covered with spines of moderate size distally, and long hooks anteriorly; two or three long basal hooks, the remaining anterior portion of the basal sector is smooth.