By the advent of molecular technologies based on Polymerase Chain Reaction (PCR) for the ribosomal gene amplification is used for the genetic identification of many organisms because they comprise both
highly conserved sequences during evolution and highly variable sequences among species.
Of these techniques, the ribosomal RNA (rRNA) gene architecture and the
highly conserved sequences of certain domains of the gene have been used to reevaluate higher level relationships within the family Phlebotominae and within genera Phlebotomus and Sergentomyia (Depaquit et al., 1988; Aransay et al., 2000).
A DNA microarray with
highly conserved sequences from >1,000 viruses was selected to screen for known and novel viruses (3).
Because primers are designed using
highly conserved sequences, the ITS primers will likely amplify in all oyster species and hence provide possible detection and identification of new or unknown species.
The goal of Alnylam's RNAi therapeutic program for influenza infection will be to employ optimized siRNAs that silence
highly conserved sequences required for viral replication across human and avian flu strains, thereby yielding a novel prevention and treatment option for world-threatening outbreaks of highly virulent strains of flu.
The use of general primers GP5 and GP6 elongated at their 3' ends with adjacent
highly conserved sequences improves human papillomavirus detection by PCR.
