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A science that deals with the distribution and activities of chemical components in tissues.



the division of histology that studies the chemical properties of animal and plant tissue.

Histochemistry seeks to elucidate the characteristics of metabolism in tissue cells and interstitial media. It studies changes in cell properties in the process of development and the connection between the work, metabolism, and renewal of mature cells and tissues. A basic principle of histochemical methodology is the bonding of a definite chemical component of cells with a stain or the formation of a stain in the process of the reaction. A number of methods (cytophotometry and luminescence and interference microscopy) are based on the physical properties of substances. Various histochemical methods determine the localization and quantity of many substances in tissue and their metabolism (tissue autoradiography), bonds with submicroscopic structure (electron histochemistry), and enzyme activity. Immunohistochemistry is a promising trend. The most precise histochemical methods, which permit investigation of cell structure, are called cytochemical methods.

The first special histochemical research was done by the French scientist F. Raspail (1825-34). Histochemistry began to develop intensively beginning in the 1940’s, when reliable methods appeared for determining proteins, nucleic acids, lipides, polysaccharides, and certain inorganic components in the cell. By means of histochemical methodology it has been possible, for instance, to show for the first time the connection of changes in the quantity of RNA with the synthesis of protein and the constancy of DNA content in the chromosome bank.


Pearse, E. Gistokhimiia: Teoreticheskaia i prikladnaia. Moscow, 1962. (Translated from English.)
Burstone, M. Gistokhimiia fermentov. Moscow, 1965. (Translated from English.)


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Demonstration of a unique population of neurons with NADPH-diaphorase histochemistry.
Lectin histochemistry of salivary glands in the Giant ant-eater (Myrmecophaga tridactyla).
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Morphology, histology and histochemistry of the digestive system of South American Catfish (Rhamdia quelen).
Structure and mucous histochemistry of the intestinal respiratory tract of the mud loach, Misgurnus anguillicaudatus (Cantor).
Consecutive sections were stained for COX following the diaminobenzidine procedure [22] and for Syt4 and Syt7 mRNAs following the standard procedure for X-ray film autoradiography of in situ hybridization histochemistry described in detail previously [16,17,23].
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Evaluation of quality indices of the gonad and somatic tissues involved in reproduction of the pearl oyster Pinctada mazatlanica with histochemistry and digital image analysis.
The 17 chapters explore simple and complex carbohydrates and glycoconjugates; methods of structural analysis of glycosaminoglycans, applications of these methods for identification of lysosomal storage diseases, and participation in the development of Lyme disease; the role of viral envelope protein glycosylation in the pathogenesis of influenza A virus; the application of lectin histochemistry in the diagnosis of lysosomal storage diseases; computational approaches for studying carbohydrate-lectin interactions in infection; the pathogenic effects of altered sialylation of specific glycoconjugates in genetic diseases; sialyltransferase regulation of cancer-associated O-glycans; and the history of pectin study, chemistry, and medicinal uses of pectin.
Chromogranin A stain was positive in polygonal cells, S-100 was positive in flattened sustentacular cells on immune histochemistry.