The activity in the liver homogenate
exhibited a significant reduction in the RC group (P=0.
The CK activity only in the homogenate
was determined spectrophotometrically with commercial CK kit (Nanjing Jiancheng Bioengineering Institute, China).
Initially, we inoculated a 200-[micro]L sample of tick homogenate
into HuH-7 cells in separate T-25 flasks, monitored the flasks daily for cytopathic effect, and harvested on day 5 or 6.
However, no significant differences in the 5-HT levels of brain homogenate
could be found between the saline treatment subgroups of MI, depression, MI + depression and control groups ( P = 0.
For GPx activity, 100 [micro]L of adipocyte homogenate
was suspended in 1.
Owing to a lack of independence in the source snails, separate analyses were run for the swab and homogenate
The nitrite/nitrate and MDA were assayed colorimetrically in homogenates
of kidney, testis, spleen and jejunum according to the methods of Green et al.
Experiments were performed on 10 dates from June to August 2011 for trials with krill homogenate
and 7 times from October 2011 to February 2012 for trials with DMS.
seems to act preferentially on substrates with single glucose units bound to alkyl chain (crocin-2 and crocin-5), while substrates that exclusively bear gentiobioside units (crocin-1 and crocin-4) are hardly affected.
Determination of oxidative markers in whole body homogenate
The level of GSH in the brain homogenate
was determined by the method of Moron et al with slight modification.
Determined at 41[degrees]C by incubating 40 [micro]l of duodenal homogenate
with 200 gl of 1 mM inositolhexaphosphoric sodium salt and 200 [micro]l of 25 mM Mg[Cl.