Determination of homovanillic
acid in urine by stable isotope dilution and electrospray tandem mass spectrometry.
Among these were hippuric acid, homovanillic
acid, uracil, 5-fluorouracil, and tryptophan.
Currently this technology is being used in laboratories for assays such as homocysteine, 17-hydroxyprogesterone, urine catecholamines, vanillylmandelic acid, homovanillic
acid, and urine metanephrines (21-25).
This is essential when investigating certain neurotransmitters, for example, the monoamine metabolites homovanillic
acid and 5-hydroxy-indoleacetic acid, for which marked concentration gradients have been found (47), but also when investigating proteins such as albumin and immunoglobulins (26).
Our laboratory recently described LC-MS/MS methods for total homocysteine (16), methylmalonic acid (17), and homovanillic
acid (18) as well as for glycosylated transferrin (19), which are currently being used in the clinical laboratory.
Low CSF concentrations of homovanillic
acid and 5-hydroxyindoleacetic acid, metabolites of dopamine and serotonin, respectively, are found in all variants of B[H.sub.4] deficiencies.
Rapid and reliable high-performance liquid chromatographic method for analyzing human plasma 5-HT, 5-hydroxyindoleacetic acid, homovanillic
acid and 3,4-dihydroxyphenylacetic acid.
The neurotransmitter metabolites 5-hydroxyindoleacetic acid (5-HIAA), homovanillic
acid (HVA), and 3-methoxy4-hydroxyphenylglycol (MHPG) in CSF and 5-HIAA, HVA, and vanillylmandelic acid (VMA) in urine were measured with HPLC and electrochemical detection, and the metabolites 3-o-methyldopa and L-dopa in CSF and dopamine, epinephrine, and norepinephrine in urine were measured with HPLC and fluorometric detection.
In contrast, no consistent differences in urine concentrations of homovanillic
acid, a major urine DA metabolite, were found after exposure to concentrated particles (Liu et al.
Two cases had incidental finding of markedly elevated homovanillic
acid (HVA) excretion in the urine.
An unequivocal pathological diagnosis from tumor tissue is made by light microscopy, with or without immunohistochemistry (IHC) or raised urine/serum homovanillic
acid (HVA) or vanillylmandelic acid (VMA).
West, "Concomitant fall in brain dopamine and homovanillic
acid in hydrocephalic rabbits," Experimental Neurology, vol.