Different molecular forces of protein-protein interactions during heating process contributed to form and maintain the gelation structures, which included ionic bonds, hydrogen bonds and hydrophobic interactions
The adsorption of alkyl sulfate surfactants onto [gamma]-[Al.sub.2][O.sub.3] was induced by electrostatic and hydrophobic interactions
. However, the hydrophobic interaction
is much stronger for STS adsorption than for SDS adsorption.
The CHP self-polymerization group is a circular or oval hydrogel NP formed by the noncovalent hydrophobic interaction
between the cholesterol groups .
Summary of purification of protease from Bacillus sp Purification Steps Total Total Specific Activity Protein Activity (U) (mg) (U/mg) Crude 11250 4050 2.78 Ammonium sulfate precipitation 5600 1500 3.73 Ultrafiltration(5 kDa) 3880 438 8.86 Phenyl-Sepharose CL-4B 663 4 165.75 hydrophobic interaction
chromatography Purification Steps Purification Yield fold (%) Crude 1 100 Ammonium sulfate precipitation 1.34 49.78 Ultrafiltration(5 kDa) 3.19 34.49 Phenyl-Sepharose CL-4B 59.62 5.89 hydrophobic interaction
chromatography Table 2.
The hydrophobic tail moiety of Rosiglitazone may also interact with helix 3, 5, 6, 7, and the [beta] strand, occupying arm II and arm III of the LBD, through van der Waals and hydrophobic interactions
which accounts for the efficiency of binding and potency of the molecule.
Also, their conformations have to be stable (e.g., stabilized by hydrophobic interaction
around aromatic residue).
in tannin-protein complexes.
Purification of monoclonal antibodies by hydrophobic interaction
chromatography under no-salt conditions.
(6) An example of entropic repulsion is when a nonionic surfactant anchors onto the latex particle via a hydrophobic interaction
, and the hydrophilic portion extends into the water phase and attains a high degree of conformational freedom.
Tannin structure variation has been shown to influence the extent of hydrophobic interaction
and it can be quantitatively measured.
The originator molecule was characterized using, among other analyses, oligosaccharide mapping, peptide mapping (by MS), size exclusion FTPLC, ion exchange HPLC, hydrophobic interaction
HPLC, SDS and IEF capillary electrophoresis, cellbased bioassay (ligand inhibition assay) and OCTET analyses of the binding kinetics of both the Fc and CDR portions of the mAb to their appropriate targets.
The primary source of protein retention on reversed phase is hydrophobic interaction
between hydrophobic parts of amino acid residues and alkyl chains in the stationary phase.