The recovery of IL-8 added to EDTA-whole blood samples, as measured in plasma or lysate, is shown in Table 1.
The concentration range of total IL-8 was determined in the lysate prepared from EDTA blood samples of 57 healthy females (median age, 35 years; range, 22-56 years) and 46 healthy males (median age, 35 years; range, 25-61 years) without any medication.
When the entire population of healthy subjects was considered, the total IL-8 values in females were only slightly higher than in males, without reaching significance (Table 2).
5, the total IL-8 concentrations found in 15 healthy women with uncomplicated pregnancies (gestational weeks 20-39) were within the range of values found in nonpregnant women with a slightly increased median (130 ng/L of blood; range, 66-208 ng/L of blood).
There has been an increasing number of reports indicating a pathogenic role of IL-8 in inflammatory and infectious diseases (8-14,24-26).
The effect of long-term storage on the IL-8 concentration of lysate samples frozen at -20 and -80[degrees]C, respectively, is shown in Fig.
The CVs calculated from the IL-8 values measured in 20 blood lysate specimens prepared from three individual EDTA blood samples (total IL-8 concentration, 74, 231, and 327 ng/L of blood) were 3.