Escherichia coli were identified based on growth MacConkey agar followed by gram staining and biochemical testing using catalase, oxidase, IMVIC test
. Further classification of isolated Gram negative strains was performed using Microbacter GNB 24E (Oxiod, UK).
was performed by using the test culture method.
Klebsiella species were identified by their characteristic growth (lactose positive, dome-shaped, mucoid and sticky colonies on MacConkey agar (Oxoid) and by biochemical tests (IMViC test
. The production of tryptophanase, sufficient amount of alcohol, acetyl methyl carbinol, and citrate utilisation were noted using the indole test, methyl red (MR) test, Voges-Proskauer (VP) test, and citrate utilization test, respectively, according to the protocol of Mackie McCartney .
The cultures giving + + - - reaction for IMViC test
were confirmed as E.
Bacterial biochemical tests included carbohydrate fermentation reactions, IMVIC test
(Indole, Methyl Red, Voges-Proskauer, Simmon's citrate), catalase test, [H.sub.2]S reducing test, and motility test.
Bacterial isolates are identified by colony characteristics, Gram staining, growth on special media and biochemical properties like catalase and oxidase test, coagulase test, motility, IMViC test
, urease test, TSI agar test.
Ampicillin-resistant a-haemolytic colonies on SBAA and Non-lactose fermenting colonies on MacConkey agar were subjected to indole, methyl red, Vogues proskauer, citrate IMVIC test
, and also inoculated on Kligler Iron Agar (KIA) slants (Oxoids).
Biochemical characterization based on various biochemical tests like catalase production, H2S production, IMViC test
, oxidase production and carbohydrate fermentation was done (Table 3).
For the bacteria that showed growth on the specialized media, certain biochemical tests were done to confirm the identity of the isolated bacteria like IMViC test
, nitrate reduction test, catalase test (Cappuccino, J.G and Sherman, 1992).
, catalase, urease, oxidase, nitrate reduction, [H.sub.2]S production and various sugar utilization tests.
All isolates showed identical reaction to IMViC tests