In IMViC test, citrate was positive and indole, methyl red and VP was negative.
In IMViC test citrate and methyl red positive, negative to indole and VP.
The cultures giving + + - - reaction for IMViC test
were confirmed as E.
Biochemical characterization based on various biochemical tests like catalase production, H2S production, IMViC test
, oxidase production and carbohydrate fermentation was done (Table 3).
For the bacteria that showed growth on the specialized media, certain biochemical tests were done to confirm the identity of the isolated bacteria like IMViC test
, nitrate reduction test, catalase test (Cappuccino, J.
, catalase, urease, oxidase, nitrate reduction, [H.
After the incubation Gram staining from colonies, and bacterial identification processes were performed for Gram-negative bacteria with oxidase and IMVIC tests
Typical colonies were subjected to biochemical confirmation by IMViC tests
, which included Indole production test, Methyl red test, Voges-Proskauer and Citrate utilization tests (17,14).
The growing colonies were taken for gram staining and IMVIC tests
which included indole voges-proskauer (VP) methyl red test and citrate tests.
Physiological characterization was done by biochemical tests such as staining reaction, oxidase test, catalase test, Imvic tests
, amylase production test, gelatin hydrolysis test, urease test, H2S production test, oxidation fermentation test of glucose for carbohydrate utilization, carbohydrate fermentation test etc.
Biochemical activities of the isolated bacteria were analyzed by different biochemical tests such as amylase production, cellulase production, degradation of pectin, hydrolysis of gelatin, casein hydrolysis, urease test, hydrogen sulfide production, carbohydrate catabolism, IMVic tests
, citrate utilization, catalaste test and oxidase test.
Characterization of selected isolates: All the Phe producers were biochemically characterized through IMViC tests