Ovaries of the slaughtered animals are the inexpensive, easy access and prime source of oocytes for the process large scale by IVM
and in-vitro fertilization (IVF) (Carolan et al., 1993).
landolti to feces of IVM-treated cattle under laboratory conditions: Following the same method as used for passive olfactometer standardization, a control treatment (80 g IVM-free feces) was placed in holes one and three while the experimental treatment (80 g feces from animals treated with 1 % IVM
) was placed in holes two and four.
Are minimal stimulation IVF and IVM
set to replace routine IVF?
The incubation temperature during IVM
had no effect on blastocyst quality, but the antioxidant supplementation into embryo culture media improved blastocyst quality (p<0.05) only in zygotes from oocytes matured at conventional temperature.
Assessment of immature GV oocytes ultrastructure after vitrification and then IVM
In conditioned-G1-PLUS (Figure 1(b)), IVM
was not significantly different from the stock media.
In addition, IVM
can be used in patients with ovarian resistance to follicular-stimulating hormone (FSH) , fertility preservation of cancer patients (particularly women with leukemia and oestrogen-sensitive tumours), and endometriosis patients undergoing extensive endometrioma excision .
The developmental potential of oocytes that had undergone Germinal Vesicle Breakdown (GVBD) or reached the MII stage via IVM
were assessed by IVF.
Nuclear maturation status of buffalo oocytes after 24 hours of maturation in IVM
medium supplemented with serum, FAF-BSA or FAF-BSA+ EGF.
Evaluacion de la estabilidad termica de los residuos de IVM
Unfortunately, very few practical examples of IVM
have been documented.
After filtration and 26 hr after the incubation in IVM
culture medium (Origio medicult IVM
medium, Denmark), the oocytes were detached (denuded) from the cumulus cell mass and 5 matured oocytes (55% maturation rate) were injected with TESA derived fresh sperm and 3 oocytes with 2 pronuclei (60% fertilization rate) were generated.