Induction of mRNA expression for IL-22 BP in PBMC after stimulation with IL-4
We found expression for mRNA of IL-22-BP in PBMCs from individuals with atopic asthma and healthy controls after incubation with IL-4 (after an incubation period of 12 hours, IL-22 BP expression hold on up to 96 hours).
We assessed no mRNA-expression of IL-22-R1 in PBMCs from individuals with asthma and healthy controls neither without stimulation nor after stimulation with PMA, IL-4, IL-9, GM-CSF and IL-22.
To determine if metal ions change the level of expression of IL-4 in antigen-activated mast cells, we sensitized mast cells with IgE and challenged them with antigen in the presence or absence of increasing concentrations of NiS[O.
Furthermore, mast cell degranulation was associated with IL-4 secretion (Figure 5), which requires de novo transcription and translation (Dastych et al.
PCR primers for rat glyceraldehyde-3-phosphate dehydrogenase (G3PDH), IL-1, IL-6, TNF-[Alpha], IL-2, and IFN-[Gamma] were purchased commercially from Clontech (Palo Alto, CA); PCR primers for rat IL-4 and IL-5 were synthesized by the core facility of the Human Studies Division at the U.
To investigate the effect of feed restriction on the balance of Th1 and Th2 cytokines, the protein levels of IL-2, IFN[Gamma], IL-4, and IL-10 in serum and in BAL fluid as well as the mRNA levels of IL-2, IFN[Gamma], IL-4, and IL-5 in pulmonary lymph nodes were assessed.
For example, the Japanese group calculates that its cells each possess about 2,800 gamma chains, nearly all of which become unavailable when the scientists add IL-2, leaving none for IL-4
Preclinical data suggest that when infused directly into a glioblastoma, IL-4
Fusion Toxin kills tumor cells but not the healthy brain cells.