affinity chromatography

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affinity chromatography

[ə′fin·əd·ē ‚krō·mə′täg·rə·fē]
(analytical chemistry)
A chromatographic technique that utilizes the ability of biological molecules to bend to certain ligands specifically and reversibly; used in protein biochemistry.
References in periodicals archive ?
Everyone is also invited to visit Bioo Scientific's poster sessions, “Flexible Detection of Aflatoxin M1 in Milk Samples Using a New Immunoaffinity Chromatography Column” and “Rapid Detection of Polyaromatic Hydrocarbons in Seafood”, which will be presented on Tuesday September 9th.
Determination of carbofuran by on-line immunoaffinity chromatography with coupled-column liquid chromatography/mass spectrometry.
Representative topics include molecular imprinting for small molecules, aptamer-based bioanalytical methods, phages as biospecific probes, upconverting phosphors for detection and identification using antibodies, mathematical aspects of immunoassays, bioanalytical test for measuring prions, environmental applications of immunoaffinity chromatography, biosensors for environmental monitoring and homeland security, immunochemical techniques in biological monitoring, microelectrode protein microarrays, and bioconjugated quantum dots for sensitive and multiplexed immunoassays.
A chapter covering post-translational modifications describes detection of phosphorylated and glycosylated proteins as well as immunoaffinity chromatography, phosphopeptide mapping, and isotopic labeling and collision-induced dissociation strategies.
Immunochemical and receptor technologies: The role of Immunoassay, Immunoaffinity chromatography, Immunosensors, and Molecularly Imprinted Polymeric Sensors
Using a different combination of chromatography and mass spectrometry modalities, other groups have focused their efforts on hyphenating relatively large-capacity, high-flow peptide immunoaffinity chromatography online with conventional LC-MS/MS (16-18).
For immunoaffinity chromatography, a commercial immunoaffinity gel (Laboratoire d'Hormonologie, C.
HCA was purified from both sources by combined use of Filtron Ultrasette, gelfiltration chromatography on columns of Sepharose CL-2B, and by immunoaffinity chromatography using columns with immobilized mouse monoclonal IgM antibodies raised against epiglycanin.
Recombinant uPAR(I-III), consisting of amino acids 1-277 (calculated mass, 30 741 Da), was produced in Chinese hamster ovary cells and purified by immunoaffinity chromatography as described previously (25).
Subsequently, the IgGSc preparations were subjected to repeated negative immunoaffinity chromatography until the purity was >98% (see below).