Everyone is also invited to visit Bioo Scientific's poster sessions, “Flexible Detection of Aflatoxin M1 in Milk Samples Using a New Immunoaffinity Chromatography
Column” and “Rapid Detection of Polyaromatic Hydrocarbons in Seafood”, which will be presented on Tuesday September 9th.
Determination of carbofuran by on-line immunoaffinity chromatography
with coupled-column liquid chromatography/mass spectrometry.
Representative topics include molecular imprinting for small molecules, aptamer-based bioanalytical methods, phages as biospecific probes, upconverting phosphors for detection and identification using antibodies, mathematical aspects of immunoassays, bioanalytical test for measuring prions, environmental applications of immunoaffinity chromatography
, biosensors for environmental monitoring and homeland security, immunochemical techniques in biological monitoring, microelectrode protein microarrays, and bioconjugated quantum dots for sensitive and multiplexed immunoassays.
A chapter covering post-translational modifications describes detection of phosphorylated and glycosylated proteins as well as immunoaffinity chromatography
, phosphopeptide mapping, and isotopic labeling and collision-induced dissociation strategies.
is an advanced purification technique
Immunochemical and receptor technologies: The role of Immunoassay, Immunoaffinity chromatography
, Immunosensors, and Molecularly Imprinted Polymeric Sensors
Using a different combination of chromatography and mass spectrometry modalities, other groups have focused their efforts on hyphenating relatively large-capacity, high-flow peptide immunoaffinity chromatography
online with conventional LC-MS/MS (16-18).
For immunoaffinity chromatography
, a commercial immunoaffinity gel (Laboratoire d'Hormonologie, C.
HCA was purified from both sources by combined use of Filtron Ultrasette, gelfiltration chromatography on columns of Sepharose CL-2B, and by immunoaffinity chromatography
using columns with immobilized mouse monoclonal IgM antibodies raised against epiglycanin.
1 mol/L ammonium bicarbonate (pH 8) before immunoaffinity chromatography
Recombinant uPAR(I-III), consisting of amino acids 1-277 (calculated mass, 30 741 Da), was produced in Chinese hamster ovary cells and purified by immunoaffinity chromatography
as described previously (25).
Subsequently, the IgGSc preparations were subjected to repeated negative immunoaffinity chromatography
until the purity was >98% (see below).