Immunofluorescence


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Immunofluorescence

A technique that uses a fluorochrome to indicate the occurrence of a specific antigen-antibody reaction. The fluorochrome labels either an antigen or an antibody. The labeled reactant is then used to detect the presence of the unlabeled reactant. The use of a labeled reactant (such as an antibody which both detects and indicates the antigen) to reveal the presence of an unlabeled one is termed direct immunofluorescence. The use of a labeled indicator antibody, which reacts with an unlabeled detector antibody that has previously reacted with an antigen, is termed indirect immunofluorescence. Substitution of a light meter for the human eye permits a quantitative measurement in immunofluorometry. See Immunoassay

Immunofluorescence

 

any set of methods of fluorescent analysis used in immunology, histochemistry, virology, bacteriology, mycology, and parasitology.

The combination of immunochemical reactions with fluorescence microscopy makes possible the detection of tissue and cellular antigens, including those involved in autoimmune diseases and in malignantly degenerated cells. The method is also useful in studying the patterns of antibody synthesis and in identifying the causative agents of many viral and microbial diseases. Specific antibodies are tagged with a fluorescent dye (for example, acridine orange) that will not alter their properties and then introduced to the specimen, so that only the parts of the specimen containing antigen will fluoresce. When the formation of antigen-antibody complexes is being investigated by immuno-fluorescence, the antibodies are tagged with a dye whose fluorescent properties will change when the antibodies combine with antigen.

REFERENCE

Immunofliurestsentsiia. Edited by J. Kubica. Moscow, 1967. (Translated from Polish.)

immunofluorescence

[¦im·yə·nō·flə′res·əns]
(immunology)
Fluorescence as the result of, or identifying, an immune response; a specifically stained antigen fluoresces in ultraviolet light and can thus be easily identified with a homologous antigen.
References in periodicals archive ?
Caption: Figure 4: Confocal microscopy of cytochrome C (red fluorescence) with mitochondrial marker (green fluorescence) double immunofluorescence of the renal tubular cells in the kidney of control and ACF adult rats.
Piazza et al., "Diagnostic accuracy of ELISA methods as an alternative screening test to indirect immunofluorescence for the detection of antinuclear antibodies.
(16) What is unique about the TRITC-labeled PG-M3 antibody is that it can be applied in a direct immunofluorescence procedure, whereas the procedure for applying the noncommercial antibody is indirect and, as described by Falini et al, (4) involves 2 techniques.
Regarding quality control methods, it is worth emphasizing that the samples were analyzed by the Provincial Reference Laboratory on Respiratory Viruses, which has over 15 years of experience with direct immunofluorescence assay (DFA) techniques, backed by more than 30 000 specimens processed during that time.
Detection of specific antinuclear reactivities in patients with negative anti-nuclear antibody immunofluorescence screening test.
The sensitivity of low nasal swabs compared with nasopharyngeal swabs for influenza detection using direct immunofluorescence and enzyme immunoassay was 86% and 87%, respectively with identical specificities of 97%.
Although the results of rapid viral testing for influenza A and B viruses were negative, influenza B infection was confirmed by viral culture and immunofluorescence antibody testing in two crew members.
Keywords: Direct immunofluorescence, Tzanck smear, pemphigus vulgaris.
Work-up for a suspected autoimmune blistering disease includes a physical exam, histology, direct and indirect immunofluorescence, and serologic tests.
M2 PHARMA-July 11, 2014-Pathology Diagnostics starts offering immunofluorescence services
Cambridge, United Kingdom, June 27, 2014 --(PR.com)-- Due to the recent rapid growth of Pathology Diagnostics Ltd (PDL), the advanced laboratory staining biomarker test portfolio of the organisation has grown at an extremely impressive rate, with the number of biomarkers validated now reaching over 370 for immunohistochemistry (IHC) and immunofluorescence (IMF).
This Sofia Analyzer and Sofia Strep A FIA combine unique immunofluorescence chemistry, advanced lateral flow technology and failure alert and fail-safe systems designed to ensure a reliable, objective, highly accurate, diagnostic result within five minutes of application of the patient's specimen, according to the company.

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