Immunofluorescence


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Immunofluorescence

A technique that uses a fluorochrome to indicate the occurrence of a specific antigen-antibody reaction. The fluorochrome labels either an antigen or an antibody. The labeled reactant is then used to detect the presence of the unlabeled reactant. The use of a labeled reactant (such as an antibody which both detects and indicates the antigen) to reveal the presence of an unlabeled one is termed direct immunofluorescence. The use of a labeled indicator antibody, which reacts with an unlabeled detector antibody that has previously reacted with an antigen, is termed indirect immunofluorescence. Substitution of a light meter for the human eye permits a quantitative measurement in immunofluorometry. See Immunoassay

Immunofluorescence

 

any set of methods of fluorescent analysis used in immunology, histochemistry, virology, bacteriology, mycology, and parasitology.

The combination of immunochemical reactions with fluorescence microscopy makes possible the detection of tissue and cellular antigens, including those involved in autoimmune diseases and in malignantly degenerated cells. The method is also useful in studying the patterns of antibody synthesis and in identifying the causative agents of many viral and microbial diseases. Specific antibodies are tagged with a fluorescent dye (for example, acridine orange) that will not alter their properties and then introduced to the specimen, so that only the parts of the specimen containing antigen will fluoresce. When the formation of antigen-antibody complexes is being investigated by immuno-fluorescence, the antibodies are tagged with a dye whose fluorescent properties will change when the antibodies combine with antigen.

REFERENCE

Immunofliurestsentsiia. Edited by J. Kubica. Moscow, 1967. (Translated from Polish.)

immunofluorescence

[¦im·yə·nō·flə′res·əns]
(immunology)
Fluorescence as the result of, or identifying, an immune response; a specifically stained antigen fluoresces in ultraviolet light and can thus be easily identified with a homologous antigen.
References in periodicals archive ?
3 The most important techniques for the investigation of patients with immunobullous disease are conventional histopathology, and confirmative tests like direct and indirect immunofluorescence.
Renal involvement in lupus is widely known as an immune complex disorder and is associated with a full-house pattern in immunofluorescence, but it must be known that without endocapillary proliferation, crescentic necrotizing pauci-immune GN may be a very rare variant of lupus nephritis.
Immunofluorescence staining with TRITC-labeled PG-M3 was tested in bone marrow and peripheral blood samples from APL cases with typical cell morphology or microgranular morphology, cases with isolated t(15;17)(q24.
Regarding quality control methods, it is worth emphasizing that the samples were analyzed by the Provincial Reference Laboratory on Respiratory Viruses, which has over 15 years of experience with direct immunofluorescence assay (DFA) techniques, backed by more than 30 000 specimens processed during that time.
Dengue virus specific and flavivirus group determinate identified with monoclonal antibodies by indirect immunofluorescence.
Detection of anti-SSA antibodies by indirect immunofluorescence.
The sensitivity of low nasal swabs compared with nasopharyngeal swabs for influenza detection using direct immunofluorescence and enzyme immunoassay was 86% and 87%, respectively with identical specificities of 97%.
Although the results of rapid viral testing for influenza A and B viruses were negative, influenza B infection was confirmed by viral culture and immunofluorescence antibody testing in two crew members.
Trask, Quantitative Immunofluorescence in Flow Cytometry, Proceedings of the VI International Conference on Immunofluorescence and Related Staining Techniques held in Vienna, Austria on April 6-8, 1978, W.
com) has produced a new Anti-NF-B monoclonal antibody (catalog number 200-301-065) validated by several assays relevant for discovery including immunofluorescence microscopy (IF), immunohistochemistry (IHC), western blotting (WB) and ELISA (E).
Contract award notice: Provision of means for the production of immunohistochemistry slides, In immunofluorescence, In situ hybridization in the hcl anatomocytopathology departments (france-lyon: Medical equipments)

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