In order to understand the binding capacity of mutated TP53 in MDA-MB-468 cell line in comparison to wild type one in MCF7 cells, cell lysates of these cells were immunoprecipitated
with antibody against BRCA1 and blotted for TP53.
proteins were resolved by SDSPAGE on 4-15% polyacrylamide gradient gels (Jule Inc.
Two non-radioactive assays were used to assess MK2 activity after various treatments using either bacterially expressed active-MK2 or myc tagged MK2 immunoprecipitated
from transiently transfected cells.
Sera from 16 of the other 26 patients immunoprecipitated
a novel autoantibody recognizing 200-kD and 100-kD proteins.
Applications can be found in the life sciences, such as removing supernatant liquids from cell cultures, DNA extractions, centrifuged magnetic beads, immunoprecipitated
proteins and from ELISA reactions.
Tb670 protein possesses protein kinase activity, the activity under normal culture conditions is modest.
Ubiquitinated NKCC1 was detected in immunoprecipitated
(IP)NKCC1 from HT29 colonic cell lines.
Strips of 21-nucleotide-long DNA subsequently are released from the immunoprecipitated
DNA to create "genomic signature tags," which subsequently are identified in the international genome database.
To assay the 1H12 antigen for carbohydrate residues, the antigen was immunoprecipitated
from frog brain homogenates, and aliquots of the immunoprecipitate were incubated in the presence or absence of deglycosylation enzymes before being probed with antibody.
To determine whether the GST-MyoV-tail fragment binds squid brain kinesin, squid brain extracts were incubated with GST-MyoV-tail fragments for 2 h at 4[degrees]C, and then the GST-labeled fragment was immunoprecipitated
with the OST antibody.
EGFR protein was immunoprecipitated
with polyclonal EGFR antibody and subjected to electrophoresis.
Kits that provide immunoprecipitated
DNA, or perform clean-up, but do not perform chromatin immunoprecipitation are also excluded.