in situ hybridization

(redirected from In situ hybridisation)
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in situ hybridization

[‚in ‚si·chü ‚hī·brəd·ə·zā·shən]
(cell and molecular biology)
A technique permitting identification of particular deoxyribonucleic acid or ribonucleic acid sequences while these sequences remain in their original location in the cell. A cell or tissue is treated with a fixative and then exposed to a labeled (by radioactivity or fluorescence) single-stranded nucleic acid probe that hybridizes with the targeted nucleic acid sequence, revealing its location on a chromosome band when the hybridized sequence is analyzed microscopically.
References in periodicals archive ?
HER-2 gene amplification by chromogenic in situ hybridisation (CISH) compared with fluorescence in situ hybridisation (FISH) in breast cancer--a study of two hundred cases.
The report further projects that in situ hybridisation will continue to be the leading cancer tissue diagnostics type.
Galena Biopharma, a biopharmaceutical company developing and commercialising innovative, targeted oncology treatments that address major unmet medical needs to advance cancer care, has received the notice of allowance of a US Patent for NeuVax, covering the use of the product alone or in combination to prevent recurrence of any HER2/neu expressing tumour having a fluorescence in situ hybridisation (FISH) rating of less than about 2.0, it was reported yesterday.
Dual-colour HER2/chromosome 17 chromogenic in situ hybridisation assay enables accurate assessment of HER2 genomic status in gastric cancer and has potential utility in HER2 testing of biopsy samples.
Comparison of automated silver enhanced in situ hybridisation (SISH) and fluorescence ISH (FISH) for the validation of HER2 gene status in breast carcinoma according to the guidelines of the American Society of Clinical Oncology and the College of American Pathologists.
The trials used Caris Molecular Intelligence's multi-technology approach, which comprised gene sequencing (next-generation [NGS] or Sanger sequencing), protein analysis (immunohistochemistry [IHC]), and gene copy number analysis (chromogenic and fluorescent in situ hybridisation [CISH and FISH]).
HER2 amplification status in breast cancer: a comparison between immunohistochemical staining and fluorescence in situ hybridisation using manual and automated quantitative image analysis scoring techniques.
M2 PHARMA-May 8, 2014-Affymetrix, Leica Biosystems unveil fully-automated RNA in situ hybridisation assays