fluorescent in situ hybridization

(redirected from In situ hybridization, fluorescence)
Also found in: Medical.

fluorescent in situ hybridization

[flə¦res·ənt in¦sit‚chü ‚hī·brə·də′zā·shən]
(genetics)
A technique in which a deoxyribonucleic acid (DNA) probe is labeled with a fluorescent dye (that can be visualized under a fluorescent microscope) and then hybridized with target DNA, usually chromosome preparations on a microscopic slide. It is used to precisely map genes to a specific region of a chromosome in prepared karyotype, or can enumerate chromosomes, or can detect chromosomal deletions, translocations, or gene amplifications in cancer cells. Abbreviated FISH.
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Introgressions will be validated by modern molecular cytogenetic techniques (genomic in situ hybridization, fluorescence in situ hybridization) and phenotyping will be carried out for disease resistance and perennial growth habit.
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