fluorescent in situ hybridization(redirected from In situ hybridization, fluorescence)
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fluorescent in situ hybridization[flə¦res·ənt in¦sit‚chü ‚hī·brə·də′zā·shən]
A technique in which a deoxyribonucleic acid (DNA) probe is labeled with a fluorescent dye (that can be visualized under a fluorescent microscope) and then hybridized with target DNA, usually chromosome preparations on a microscopic slide. It is used to precisely map genes to a specific region of a chromosome in prepared karyotype, or can enumerate chromosomes, or can detect chromosomal deletions, translocations, or gene amplifications in cancer cells. Abbreviated FISH.