IMVIC is an acronym that stands for four different tests; Indole test
, Methyl red test, Voges-Proskauer test, Citrate utilization test to obtain the results of these four tests, three test tubes are inoculated: tryptone broth (indole test
), methyl red/Voges-Proskauer broth (MR-VP broth), and citrate.
The principal biochemical tests performed for this purpose include, lactose fermentation test (LAC), indole test
(IND), methyl red test (MR), Voges-Proskauer test (VP), urease test (URE), catalase test (CAT), aerobic and anaerobic test (Ae/An).
Result of biochemical characterization (A) Amylase production test (B) Carbohydrate catabolism test (C) Casein hydrolysis test (D) Catalase test (E) Citrate utilization test (F) Gelatin hydrolysis test (G) H2S production test (H) Indole test
(I) MR test (J) Oxidase test (K) Urease test (L) VP test
identified on the basis of biochemical tests whose antibacterial activity measured through inhibition zone was of 2 mm found to be negative for indole test
(green layer at the top of test tube) and starch agar test.
These were used to obtain additional cultures and further characterize the bacterial colonies by Gram staining, motility, oxidase, and indole tests
No Test Results 1 Indole Test
(+)ve 2 Methyl Red Test (-)ve 3 Voges Proskauer Test (-)ve 4 Citrate Utilization Test (+)ve 5 Urease Test (+)ve 6 Gram's Staining Gram Negative 7 Motility Motile 8 Shape Cocci 9 Nature Clustered Table 2: RSM-FCCCD Experimental Design Std Run Temperature ([degrees]C) pH Time (h) OD at 410nm 1 4 60 8 2 0.
Salmonella colonies were identified using standard biochemical tests including fermentation of glucose negative urease reaction lysine decarboxylase negative indole test
H2S production and fermentation of dulcitol.
For further confirmation biochemical tests were performed such as indole test
, catalase test, triple sugar iron test and MRVP test.
coli to produce cream-coloured colonies (although rarely) (17); and false positive spot indole test
They included: Indole test
, methyl red test (MR), citrate utilization, catalase test, oxidase test, coagulase test, oxidation--fermentation test, fermentation of sugars, and motility test.
Motility test and indole test
were carried out by inoculating the culture into the motility indole lysine agar slant procured from Hi-Media Pvt.
coli, with confirmation requiring a simple indole test