Analysis of the mtrR gene and its promoter identified a single nucleotide (A) deletion in the inverted repeat
of the promoter region, and a single amino acid substitution at position H105 (H [right arrow] Y).
dentatum only contained 2 copies of one 11-nt direct repeat (A1 and A2) and one 10-nt inverted repeat
(B and B rev comp).
As some of the MLPA probes bind to multiple sites (due to the presence of a large inverted repeat
on the Y-chromosome) the deletions shown in table 1 are just one of the several possibilities in each case.
Transfer of the inverted repeat
construct into the shuttle vector, pArt (Gleave, AP.
One of the group of Cham 1 sequences (e.g., C7.1.2) has all five characteristics of Stowaway elements: a conserved 11-bp terminal inverted repeat
, small size (80-323 bp), AT rich, strong target site preference for TA, and the potential to form DNA secondary structures (Bureau and Wessler, 1994).
The cryptomonad genome contains a short (4.9 kb) inverted repeat
containing the rRNA cistrons, 30 tRNA genes, 44 genes encoding proteins for photosynthesis, 44 ribosomal protein genes (26 large subunit and 18 small subunit), 5 genes for biosynthetic function, 19 genes involved in gene expression (including 3 hypothetical chloroplast reading frames or ycfs), 25 additional ycfs, and 9 open reading frames (ORFs) larger than 50 amino acids.
Only PCB contained one 13 nt complete direct repeat (J1 and J2) and one 12 nt incomplete inverted repeat
(W and W reverse complement), which were missing the intervening sequences.
ISSR-PCR markers used to analyze polymorphism of DNA fragments flanked by inverted repeats
of microsatellite loci based upon the standard protocol (Zietkiewiez et al., 1994).