isoelectric focusing


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Related to isoelectric focusing: isoelectric point

isoelectric focusing

[¦ī·sō·i′lek·trik ′fō·kəs·iŋ]
(physical chemistry)
Protein separation technique in which a mixture of protein molecules is resolved into its components by subjecting the mixture to an electric field in a supporting gel having a previously established pH gradient. Also known as electrofocusing.
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The protein fractions showing considerable variations were subjected to isoelectric focusing.
Quantification of hemoglobin A1c by capillary isoelectric focusing.
Quantification of hemoglobin variants by capillary isoelectric focusing.
Detection of oligoclonal IgG bands (OB) in CSF by isoelectric focusing (IEF) is the most sensitive (approximately 95% in Western patients) laboratory test for MS and the best method to show local intrathecal IgG-synthesis (Figure 1).
Proteins migrate in isoelectric focusing gels to their neutral isoelectric point (pI), where the protein has zero net charge.
We fractionated cells by preparative isoelectric focusing in a pH gradient of 3-10 and bioassayed the resultant fractions in test diets to determine their effects on egg produc tion.
The new system, which fully automates all processes from isoelectric focusing to polyacrylamide gel electrophoresis to detection, can perform two-dimensional electrophoresis of proteins in about one hour.
This protein was found to be present in numerous cell cultures so I purified to homogeneity by preparative isoelectric focusing and SDS polyacrylamide gel electrophoresis this protein from human embryonic kidney cells (i.
The technique gives simultaneous focusing and separation of differently charged analytes in a manner analogous to isoelectric focusing of proteins, but is much simpler to implement than isoelectric focusing and has the additional advantage of working with any charged analyte, rather than just proteins.
Isoelectric focusing (IEF) in agarose gels revealed species-specific protein profiles in the ten control species.