isoelectric focusing


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Related to isoelectric focusing: isoelectric point

isoelectric focusing

[¦ī·sō·i′lek·trik ′fō·kəs·iŋ]
(physical chemistry)
Protein separation technique in which a mixture of protein molecules is resolved into its components by subjecting the mixture to an electric field in a supporting gel having a previously established pH gradient. Also known as electrofocusing.
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Isoelectric focusing determined that the isoforms, named GST1 and GST2, have pI values of 4.5 and 6.2, respectively.
Each of the eluted protein was freeze dried and reconstituted in the buffer when used for isoelectric focusing. 10 uL solution D (10 % w/v sodiumdodecyle sulfate in 2.3 % w/v dithioerythreitol) was added to 60 uL eluted protein solution, mixed and heated at 95degC for 5 minutes.
The optimized two-dimensional electrophoresis technology for ramie consists of the optimized TCA/acetone extraction method, protein purification using a four-fold volume of pre-cooled acetone after the cleavage reaction; isoelectric focusing for 50000 VH.
For getting an idea about separation accuracy during different purification processes all fractions were tested with inhibitor activity by IEF technique which could be use also as a detection method according to isoelectric focusing point.
Quantification of hemoglobin A1c by capillary isoelectric focusing. Annual meeting of the American Association of Clinical Chemistry, Atlanta, Ga, 1997, 1997.
This hemoglobin variant was detected by isoelectric focusing (IEF) moving just ahead of Hb A.
Quantification of hemoglobin variants by capillary isoelectric focusing. Clin Chem.
* Detection of oligoclonal IgG bands in CSF by isoelectric focusing is an important part of the diagnostic process in MS (Class I evidence).
Capillary isoelectric focusing (clEF) is another accurate method where the sensitivity of detection of capillary electrophoresis is combined with the automated sampling and data acquisition of HPLC (5,6).