isotopic label

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Related to Isotopic labeling: radiolabeled

isotopic label

[¦ī·sə¦täp·ik ′lā·bəl]
(chemistry)
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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Metabolic Isotopic labeling, Polymerase Chain Reaction (PCR), Gel electrophoresis, Macromolecule blotting and probing, Southern blotting, Northern blotting, Western blotting, Eastern blotting, DNA microarray, Allele Specific Oligonucleotide (ASO) and Antiquated technologies.
Topics include using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition, stable isotope labeling by amino acids in cell culture for quantitative proteomics, quantification of proteins and metabolites without isotopic labeling, an isotope coding strategy for proteomics involving both amine and carboxyl group labeling, tandem mass spectrometry in the detection of inborn errors of metabolism for newborn screening, computational analysis of quantitative proteomics data using stable isotope labeling, and quantitative proteomic analysis of phosphotyrosine-mediated cellular signaling networks.
Most of these studies have described approaches for relative quantification by use of differential isotopic labeling of paired specimens (4).
Another feature of the experiment is the presentation of isotopic labeling by using d6-acetone.
A chapter covering post-translational modifications describes detection of phosphorylated and glycosylated proteins as well as immunoaffinity chromatography, phosphopeptide mapping, and isotopic labeling and collision-induced dissociation strategies.