Unlike epidermolytic ichthyosis, which is caused by germline mutation in KRT1 or KRT10
, somatic mutations in these genes have not been found in epidermolytic acanthomas.
Se estudiaron 31 genes de queratina reportados para ovinos, cabras y humanos entre los cuales tenemos 12 genes del epitelio de tipo I (KRT9, KRT10
, KRT12, KRT13, KRT14, KRT15, KRT16, KRT17, KRT18, KRT19, KRT20 y KRT25), 13 genes del epitelio de tipo II (KRT1, KRT2, KRT3, KRT4, KRT5, KRT6a, KRT6b, KRT6c, KRT7, KRT8, KRT71, KRT79 y KRT80), 3 genes de la fibra de tipo I (KRT31, KRT32 y KRT40) y 3 genes de la fibra de tipo II (KRT81, KRT82 y KRT83) (Zhidong et al.
Immunohistochemistry was performed by using primary Abs against CD3 (Dako, Glostrup, Denmark), Ly6G, CD11c, and CD11b (BD Biosciences), Ki67 (Novocastra, Newcastle upon Tyne, UK), KRT10
(Covance), phospho-STAT3 (Tyr705) and phosphoSTAT1 (Tyr701) (both from Cell Signaling), IL-17A (R&D Systems) and IL-22 (Novus Biologicals, Oakdille, Canada), and immunoreactivities developed with secondary biotinylated mAbs and staining kits (Vector Laboratories, Burlingame, CA, USA).
The composition excludes materials that complex with the artichoke leaf extract and the carob fruit extract, and wherein the artichoke leaf extract and the carob fruit extract are present at an amount to provide a synergistic increase in the up-regulation of at least one epidermal-associated gene selected from the group consisting of KRT2, KRT6A, CLDN1, LOR, FLG, IVL, KRT10
, AQP3, and KRT14 according to an ex vivo tissue analysis.
1997), and it has been shown to inhibit KRT10
(keratin 10) mRNA and protein expression in epidermal keratinocytes in vitro (Poumay et al.