Bean regenerants with kanamycin
were selected during three to five weeks for stable transformation.
Overexpression of the chromosomally encoded aminoglycoside acetyltransferase eis confers kanamycin
resistance in Mycobacterium tuberculosis.
pneumoniae isolates to gentamicin, kanamycin
, tobramycin, and amikacin was 34.6, 43.8, 33.8, and 14.6%, respectively.
tumefaciens strain GV3101 harboring pCAMBIA-1301-CsEXP10 was grown on Yeast Extract Peptone (YEP) medium supplemented with 50 mg L-1 kanamycin
and 50 mg L-1 rifampicin on incubator shaker (28 +-1degC, 250 rpm) for 16 h.
amylovora were grown on NGA with added kanamycin
(Km) at 28degC.
The next most frequent resistance isolates were resistance to erythromycin, cefoxitin, clindamycin, ampicillin, amoxicillin, tetracycline, and kanamycin
was found to be maximum sensitive (Table 3) for Ciprofloxacin (100%) followed by Norfloxacin (85.71%), Tobramycin (85.71%), Ceftriaxone (71.42%), Amikacin (71.42%), Amoxycillin (71.42%), Ampicillin (71.42%) and Enrofloxacin (57.14%) but less sensitive to Neomycin, Streptomycin, Chloramphenicol, Kanamycin
, Cefoperazone and Cephalexin.
Resistance to the aminoglycosides was 6% to kanamycin
and 0% to gentamicin.
LaPiana, "Pharmacokinetics of kanamycin
following intramuscular administration," Journal of Pharmacokinetics and Biopharmaceutics, vol.
The recombinant clones were selected on LB agar containing ampicillin (75 [micro]g/ mL) and kanamycin
Future studies should use antimicrobial agents such as cephalosporins, fluoroquinolones, and trimethoprim, which are now more commonly in use over some of the redundant (oxytetracycline, chlortetracycline, and tetracycline) antimicrobials or ones that are no longer commonly used to treat clinical infections (kanamycin
, chloramphenicol, and nalidixic acid) to better determine the pollution source.
As compared to standard antifungal disc kanamycin
, these complexes showed minor activity against selected fungal strains at 50 u l.