BCKD-Branched Chain Alpha Keto Acid
dehydrogenase complex, MSUD-Maple Syrup Urine Disease
e]) and indicates the presence of other non-measured weak anions such as keto acids
, sulphates, urates, citrate, pyruvate, acetate and gluconate (2,3,13,15,21-26).
The choice of derivatizing reagent which should convert polar keto acids to nonpolar compounds for reproducible elution separation and determination from reversed phase HPLC.
The keto acids contain a carbonyl group on adjacent carbon atom to carboxylic acid in PYR KB MKBA KG K3MVA K4MVA and PPY and reacted with DDB to form cyclic ring structure as substituted tetramethyl dihydro-pyrazinol (Fig.
Blood sample (5 ml) collected from the diabetic patient was treated as determination of a- Keto acids from Serum.
The urine sample (2 mL) in duplicate from diabetic patient was treated as Determination of a- Keto acids from Urine.
Gas chromatography of branched-chain keto acids
analyzed as their silylated oxime derivatives.
In fact, a previous study demonstrated its usefulness in improving precision of keto acids
The aim of the present study was to separate and identify more organic acids, including new groups of aromatic acids, amino acids, and keto acids
with relevance for diagnostic purposes.
Effect of ethanol and keto acids
on the iMg electrodes.
One would expect that if 4-HPP was present in the tissues, reduction of a considerable amount of keto acid
to 4-HPL by lactic acid dehydrogenase (11) and/or aromatic [alpha]-keto acid
reductase (12,13) would take place.
Depleted tissue stores of TPP and thiamin deficiency are interconnected, and in animal studies thiamin deficiency is correlated with large reductions in the level of mitochondrial, plasma and liver TPP, resulting in a major influence on the activities of the keto acid