El reactor consistio en un recipiente de vidrio marca Kimax
de 2 L de capacidad hermeticamente sellado, inicialmente con 1.250 L de agua de despulpado y 0,250 L de fluido ruminal con 88,9 g/L de SSV.
vorax microstomal cells were washed three times in inorganic medium and suspended in one half ml of inorganic medium contained in 15 x 1 cm Kimax
Two 10 ml samples of the AP preparation were placed in 20 ml Kimax
centrifuge tubes and centrifuged in an IEC HNSII centrifuge (Damon/IEC Division, Needham HTS, Massachusetts, USA) at 3 000 rpm for 10 minutes.
For the derivatization reaction, an aliquot of 50 [micro]L of supernatant was removed and added to 16 Kimax
glass test tubes (125 mm; Kimble/Kontes, Vineland, NJ) containing 1.5 mL acetylacetone reagent (20% acetylacetone, 20% ethanol in deionized water, vol/vol) and 450 [micro]L 37% formaldehyde.
Ail tubes are constructed of KIMAX
borosilicate glass with two grades available: KG-33 glass is the lowest thermal expansion glass tubing offering the highest degree of thermal shock resistance for high temperature applications; and N-51A is low thermal expansion glass tubing to accommodate ambient temperature applications.
The glassware, high performance, high quality KIMAX
borosilicate glass, is durable when properly cared for.
Manufactured from proprietary KIMAX
N-51A borosilicate glass, ten sizes of 5-mm Thrift NMR tubes are now available.