First detection of Leishmania major in peridomestic Phlebotomus papatasi from Isfahan province, Iran: comparison of nested PCR of nuclear ITS ribosomal DNA and semi-nested PCR of minicircle kinetoplast
The first evidence of the existence of kinetoplast
came over a century ago after the discovery of a basophilic granule located near the base of trypanosome's flagellum.
Nuclear and kinetoplast
DNA were stained with DAPI and shown by blue fluorescence.
Ray, "Localization of a type II DNA topoisomerase to two sites at the periphery of the kinetoplast
DNA of Crithidia fasciculata," Cell, vol.
f, flagellum; m, mitochondria; n, nucleus; k, kinetoplast
. Scale bar = 1 [micro]m.
Luteolin, an abundant dietary component is a potent anti-leishmanial agent that acts by inducing topoisomerase II-mediated kinetoplast
DNA cleavage leading to apoptosis.
The present study was designed to report the prevalenceof Trypanosoma brucei in blood samples of donkeys (n=283) collected from Dera Ghazi Khan District in Punjab (Pakistan) through PCR amplification of their maxicircle kinetoplast
DNA (kDNA) during four seasons in 2013 and to demonstrate the effect of this parasite on complete blood count (CBC) and selected parameters of serum.
(4,18-22) The target DNAs for these studies have been kinetoplast
DNA, rDNA (internal transcribed spacer [ITS]), repetitive nuclear DNA, mini-exon genes, and microsatellite DNA.
protozoa are characterized by a single mitochondrion near the flagellum which contains DNA in a small compartment called a kinetoplast
Most of the Excavata organisms (6 species, 3 from the genus Leishmania and 3 belonging to the genus Trypanosoma) belong to a subgroup of the Euglenozoa called the Kinetoplastea, which are characterised, so far, by the presence of a mass of DNA associated with their flagellar bases (kinetoplast
) (Adl, et al., 2005).
In addition, ultrastructural analyses showed remarkable alterations in the morphology of the Golgi complex, kinetoplast
, endoplasmic reticulum and plasma membrane of trypomastigotes, confirming a pattern consistent with necrotic cell death.
and Trypanosoma cruzi, and is characterized by the presence of a kinetoplast
(Shapiro and Englund, 1995).