Detection of specific sequence motifs concerned the following signal sequences: the Kozak sequence
- at position g.
sup] Rs28383345 was located at -2 site of the Open reading frame (ORF) of HLA-DQA1 , which was the motif of the Kozak sequence influencing translational efficacy.
sup] As Kozak sequence plays a major role in the initiation of the translation process in eukaryotic cells, the minor allele of rs28383345 might be able to affect the translation of HLA-DQA1 .
Rajabi Memari; Shahid Chamran University of Ahvaz), contained restriction site for XhoI (bold) and Kozak sequence (underlined).
Kozak sequence lies within the 5' untranslated region of eukaryotic genes and directs translation of mRNA by causing the ribosome to pause and recognize the ATG codon.
Further optimisation processes were conducted including codon/RNA optimisation, and addition of a Kozak sequence
and IgE leader sequence to enhance the expression of the vaccine in humans.
The authors designed primers at two ends of the c-FLIP(L) open reading frame, and inserted Bg/II Restriction Enzyme site in the upstream prime and four protective bases before ATG, meanwhile they inserted a Kozak sequence, which could increase inserted gene expression level, in the eukaryotic cell.
Therefore the Kozak sequence was introduced after upstream primer's Bg/II site, to make sure that c-FLIP(L) gene was highly expressed in recombinant plasmid.
The presence of the -5C allele increases the mean level of GPIb[alpha] on the platelet plasma membrane (roughly a 50% increase in homozygous individuals and a 33% increase in heterozygous individuals), presumably because it enhances the interaction of the cellular protein translation machinery to the adjacent Kozak sequence
Variability in these regions, especially in the so-called KOZAK sequence
around the start codon (33), may influence efficiency of translation initiation and, thus, protein expression and virus production.
First esat6 gene construct was made in pND vector without Kozak sequence
upstream the gene, second construct was made in pcDNA3.
Thc introduction of complete Kozak sequence
in the beginning of GRA5 gene would allow optimum initiation of protein expression (32).