In their study on Tor putitora, they reported that maximum Ca accumulation and minimum Fe accumulation caused changes on annulus formation and the aluminium accumulation also caused brittleness in the margin of scales., Rishi and Jain (1998) reported that fish group (Cyprinus carpio) which was exposed to different level (14.5, 29, 43.5 and 58 mg L-1 test
solution) of cadmium concentration, lepidont and circulus were damaged in both anterior and posterior parts of the scales.
The samples were scored after 15 min following the addition of the L-1 test solution.
The L-1 test solution was prepared by adding 1.25 mL of L-1 buffer, 0.25 mL of methylene blue, 0.25 mL of distilled water, and 0.25 mL of substrate solution for each sample.
The average reaction time for the L-1 test was [approximately equals] 3 min for the analysis of individual seed chips or a bulk of chips from four seeds from an individual plant.
The use of individual seed chips for the three tests and the use of a bulk sample of seed chips for the L-1 test proved successful.