When the L-2 test was conducted, the portion of the seed that lacked the embryonic axis was split into three pieces.
Samples were left undisturbed for at least 10 min for the L-1 or the L-2 test or at least 30 min for the L-3 test.
2 [micro]L of polyoxyethylenesorbitan mono-laurate (Tween 20) per sample for the L-1 or L-2 test or 2.
The average reaction time for the L-2 test was [approximately equals] 5 min for individual seed chips, similar to the time reported by Suda et al.
The amount of time the seed chips were allowed to soak in water before the addition of the test solution was three times greater than the time allowed for the L-1 or L-2 test.
Because of the tight coupling-phase linkage between lx1 and lx2, the use of the L-2 test is optional because a seed or plant that is lx1lx1 should be lx2lx2, if it was derived from a triple-null X normal cross.
The dye for the L-1 and L-2 tests is methylene blue and for the L-3 test is [Beta]-carotene.